Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,108,507	T→G	intergenic (‑5/‑272)	metJ ← / → metB	transcriptional repressor protein MetJ/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,108,507	0	T	G	100.0%	115.7 / NA	29	intergenic (‑5/‑272)	metJ/metB	transcriptional repressor protein MetJ/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/15);  total (14/15)

ACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAT  >  REL606/4108438‑4108579                                                                      |                                                                         aCTTGTTCTCTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGCGAGACTTaa                                                                    >  1:43043/1‑76 (MQ=255) aCTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTaa                                                                    <  1:590809/76‑1 (MQ=255)   ttGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATc                                                                  >  2:122065/1‑76 (MQ=255)       tCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCctct                                                              <  1:768457/76‑1 (MQ=255)        cACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCtt                                                             <  1:909383/76‑1 (MQ=255)          ctctTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCg                                                           >  1:760187/1‑76 (MQ=255)          ctctTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCg                                                           >  1:760163/1‑76 (MQ=255)            cttcttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTc                                                         <  2:936180/76‑1 (MQ=255)            cttcttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTc                                                         >  2:239809/1‑76 (MQ=255)              tcttGCCGAGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCaa                                                       >  1:53307/1‑76 (MQ=255)               cttGNCGTGCTCAGCGTATGGGCGGATATATACGCCGCTNCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAAt                                                      <  1:177853/76‑1 (MQ=255)               cttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAAt                                                      <  1:391313/76‑1 (MQ=255)                ttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATa                                                     <  1:986576/76‑1 (MQ=255)                  gCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATaaa                                                   >  2:562815/1‑76 (MQ=255)                          cAGCTTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGagacc                                           >  2:1424957/1‑76 (MQ=255)                              gTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGagaccagac                                       >  2:492649/1‑76 (MQ=255)                               tATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                      <  1:946962/76‑1 (MQ=255)                                aTGGGCTGATATAATCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                                     <  1:426157/76‑1 (MQ=255)                                       gATATATTCGCCGCTCCATTCAGCCATGAGAGACTTNATCCTCTTCGACAATCAATTGAGACCAGACCACANAGtt                              >  1:181920/1‑76 (MQ=255)                                          atatTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt                           >  1:234100/1‑76 (MQ=255)                                           tatTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg                          <  2:1122247/76‑1 (MQ=255)                                            atTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACTAGACNACACAGTTGAtgt                         >  2:1323197/1‑76 (MQ=255)                                            atTCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgt                         <  1:394994/76‑1 (MQ=255)                                              tCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTgg                       <  1:902749/76‑1 (MQ=255)                                              tCGCCGCTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTgg                       <  1:384160/76‑1 (MQ=255)                                                 ccgcTCCATTCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa                    >  1:917094/1‑76 (MQ=255)                                                          tCAGCCATGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTa           <  1:637044/76‑1 (MQ=255)                                                                aTGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCg     <  1:835400/76‑1 (MQ=255)                                                                aTGAGAGACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCg     >  2:704795/1‑76 (MQ=255)                                                                  gagagaCTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCa   <  1:122065/76‑1 (MQ=255)                                                                   agagACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAt  <  1:238571/76‑1 (MQ=255)                                                                      |                                                                         ACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAT  >  REL606/4108438‑4108579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑