Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,009	C→G	D411H (GAT→CAT)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,009	0	C	G	100.0%	184.8 / NA	46	D411H (GAT→CAT)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/23);  total (23/23)

AGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTT  >  REL606/1304936‑1305080                                                                          |                                                                        agagTAATCTCGAAATTAAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTGATCATNGa                                                                       >  2:1364301/1‑76 (MQ=255)    gTAATCTCGAAATTTAGCCATANNCTCCGCATATNNGGGTTAACCGTGCCGACCAGACTTAGTTCACCATGGACCa                                                                    <  1:1298158/76‑1 (MQ=255)     tAATCTCGGAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACTATGGACCAg                                                                   >  1:51963/1‑76 (MQ=255)      caTCTCGAAATTTAGCCACAGACTCCGCATCTCAAGTCTAACGGTGCCAACCAGACTTAGTTCACCATCAACCAGc                                                                  >  1:282785/2‑76 (MQ=255)      aaTCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGc                                                                  >  1:282781/1‑76 (MQ=255)      aaTCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGATCACCATGGACCAGc                                                                  >  1:282783/1‑76 (MQ=255)      aaTCNCGAAATTTAGCCACAGACGCCGCGTAGCAAGGTTNACGGTGCCAACCAGACTTAGTTCACCATGGACCAGc                                                                  <  1:172579/76‑1 (MQ=255)       aTCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCa                                                                 <  1:804091/76‑1 (MQ=255)        tctcGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCAc                                                                <  2:1040108/76‑1 (MQ=255)           cGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGct                                                             >  2:1263873/1‑76 (MQ=255)           cGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGct                                                             >  1:1170446/1‑76 (MQ=255)             aaaTTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGctct                                                           <  2:872705/76‑1 (MQ=255)               aTTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTg                                                         <  2:331322/76‑1 (MQ=255)                  tAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTa                                                      >  2:945841/1‑76 (MQ=255)                  tAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTa                                                      <  1:45557/76‑1 (MQ=255)                     ccACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGc                                                   <  2:392806/76‑1 (MQ=255)                       acaGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAg                                                 >  2:1076877/1‑76 (MQ=255)                        caGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGt                                                >  2:1270455/1‑76 (MQ=255)                          gACTCCGCATATCAAGGTTAACGGTGCCAACCNGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCANTaa                                              >  2:1349424/1‑76 (MQ=255)                            cTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAAcc                                            >  1:324081/1‑76 (MQ=255)                             tCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAAccc                                           >  1:1184830/1‑76 (MQ=255)                             tCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAAccc                                           >  1:145280/1‑76 (MQ=255)                             tCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAAccc                                           >  1:389427/1‑76 (MQ=255)                                gCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGcc                                        <  2:1063926/76‑1 (MQ=255)                                gCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGcc                                        >  1:338224/1‑76 (MQ=255)                                 cATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                       >  2:31466/1‑76 (MQ=255)                                  atatCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCtt                                      >  2:720217/1‑76 (MQ=255)                                   tatCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                     <  2:300402/76‑1 (MQ=255)                                    agcAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa                                    <  1:876394/74‑1 (MQ=255)                                       aaGGCTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                 >  2:1425888/1‑76 (MQ=255)                                         ggTTAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                               <  2:715225/76‑1 (MQ=255)                                            tAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATa                            >  2:1130551/1‑76 (MQ=255)                                            tAACGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATa                            <  1:216396/76‑1 (MQ=255)                                             aaCGGTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                           >  2:1273880/1‑76 (MQ=255)                                                ggTGCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtt                        <  2:608779/76‑1 (MQ=255)                                                  tGCCAACCAGACTTAGTTCACCATGGACCTGCACGCGCTTGGTATGCAGTAACCCGCNTTCAAACTGATAAAtttt                      <  1:1383072/76‑1 (MQ=255)                                                   gCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                     <  1:533634/76‑1 (MQ=255)                                                   gCCAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                     <  1:1395302/76‑1 (MQ=255)                                                     cAACCAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                   <  2:396095/76‑1 (MQ=255)                                                        ccAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAcccc                <  2:658934/76‑1 (MQ=255)                                                         cAGACTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCcca               <  1:629877/76‑1 (MQ=255)                                                            aCTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagca            <  2:433539/76‑1 (MQ=255)                                                             cTTAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcag           >  2:447938/1‑76 (MQ=255)                                                               tAGTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc         >  2:740242/1‑76 (MQ=255)                                                                 gTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccaggacccag       <  1:89328/76‑1 (MQ=255)                                                                 gTTCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccag       >  1:263233/1‑76 (MQ=255)                                                                   tCACCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATNTTAACCccagcagccagca     <  1:1348967/76‑1 (MQ=255)                                                                     aCCATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt   >  2:93231/1‑76 (MQ=255)                                                                      ccATGGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt  >  2:621696/1‑76 (MQ=255)                                                                          |                                                                        AGAGTAATCTCGAAATTTAGCCACAGACTCCGCATATCAAGGTTAACGGTGCCAACCAGACTTAGTTCACCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTT  >  REL606/1304936‑1305080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑