Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	114.5 / NA	31	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/12);  total (19/12)

TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588                                                                          |                                                                         tGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtac                                                                        >  1:994748/1‑76 (MQ=255)  gAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTtaca                                                                       <  2:607001/76‑1 (MQ=255)   aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAATGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      <  2:1230786/76‑1 (MQ=255)     tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  1:1281155/1‑76 (MQ=255)     tCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATcc                                                                    >  2:1109441/1‑76 (MQ=255)      ccccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcg                                                                   <  2:369868/76‑1 (MQ=255)             aaaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTg                                                            >  2:1277191/1‑76 (MQ=255)              aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:1415602/1‑76 (MQ=255)               aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGcc                                                          >  2:1009517/1‑76 (MQ=255)               aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCAATTTGca                                                          >  2:1373744/1‑75 (MQ=255)                        cGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTgg                                                 >  1:829740/1‑76 (MQ=255)                            caacaaGTATCTGGGTAGTGACTACGTNGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCa                                             >  1:1433610/1‑76 (MQ=255)                             aacaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAg                                            <  1:1029810/76‑1 (MQ=255)                              acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           <  1:484303/76‑1 (MQ=255)                                   tATCCGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  2:1419842/1‑76 (MQ=255)                                        gggTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAag                                 >  2:314952/1‑76 (MQ=255)                                              tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGcc                           >  2:483918/1‑76 (MQ=255)                                              tGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGANGANCAGTGGCTCAGCTGNCAAAAAGAGTGcc                           >  2:1304061/1‑76 (MQ=255)                                               gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          <  2:988014/76‑1 (MQ=255)                                               gACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCg                          >  1:902916/1‑76 (MQ=255)                                                 cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCANAAAGAGTGcctgc                        >  1:190658/1‑73 (MQ=255)                                                 cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        <  1:150099/76‑1 (MQ=255)                                                             aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:1106599/76‑1 (MQ=255)                                                             aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCCCAGCTGCCAAAAAGAGTGCCGACTCTACCTccacc            <  1:66952/76‑1 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCATAAAGAGTGCCGACTCTACCTCCACCaa          <  1:1429250/76‑1 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  2:1134471/76‑1 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  1:362472/76‑1 (MQ=255)                                                               ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          <  1:247611/76‑1 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         >  2:894102/1‑76 (MQ=255)                                                                cAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAg         >  1:1159103/1‑76 (MQ=255)                                                                cAGCGTCGGTTACACCCGCGATTTACCGGCCNGTGGCTCACCTGCCNAAAAGNGTGCCGACTCTACCTCCACCAAg         <  2:190652/76‑1 (MQ=255)                                                                 aGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGa        <  2:642061/76‑1 (MQ=255)                                                                   cGTCGGATACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAATAGTGCCGACTCTACCTCCACCAAGACg      <  1:1419842/76‑1 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTNTACCTCCACCAAGACGGCt   <  1:1340398/76‑1 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   >  2:422026/1‑76 (MQ=255)                                                                      cGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCt   >  1:1420911/1‑76 (MQ=255)                                                                       ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  >  1:1064681/1‑76 (MQ=255)                                                                          |                                                                         TGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329443‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑