Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,512,503	C→T	intergenic (‑245/‑88)	yhhX ← / → yhhY	predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,512,503	0	C	T	100.0%	221.6 / NA	57	intergenic (‑245/‑88)	yhhX/yhhY	predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

GTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTAC  >  REL606/3512430‑3512572                                                                          |                                                                      gTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCa                                                                     <  1:319672/76‑1 (MQ=255) gTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCa                                                                     <  1:1391764/76‑1 (MQ=255)  tCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCaa                                                                    <  2:697499/76‑1 (MQ=255)   cGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAac                                                                   <  2:362652/76‑1 (MQ=255)     tGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAacac                                                                 >  1:1041745/1‑76 (MQ=255)     tGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGATCAacac                                                                 >  1:1041753/1‑76 (MQ=255)      gCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACt                                                                <  1:630561/76‑1 (MQ=255)       cTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACtt                                                               <  2:516916/76‑1 (MQ=255)        tttCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACttt                                                              >  1:556439/1‑76 (MQ=255)         ttCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACtttt                                                             >  1:893374/1‑76 (MQ=255)           cAGGTTCTCCGCNAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTg                                                           >  1:21267/1‑76 (MQ=255)           cAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTg                                                           >  2:425579/1‑76 (MQ=255)            aGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGc                                                          <  2:1288686/76‑1 (MQ=255)             ggTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCa                                                         <  1:1137432/76‑1 (MQ=255)             ggTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCa                                                         <  1:499238/76‑1 (MQ=255)              gTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCaa                                                        >  1:445361/1‑76 (MQ=255)                   ccGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCaaaaaaa                                                   <  2:305478/76‑1 (MQ=255)                   ccGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCaaaaaaa                                                   >  1:428135/1‑76 (MQ=255)                   ccGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCaaaaaaa                                                   >  1:460957/1‑76 (MQ=255)                      cgAGGGTCTTCCTGATCGCGAGACAGTAATACTCATTCTANNCCGCACTTGTNNATCACTTTTTGCAAAAAAATGc                                                >  1:1290465/1‑76 (MQ=255)                      cgAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGc                                                <  2:321069/76‑1 (MQ=255)                      cgAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGc                                                >  2:871547/1‑76 (MQ=255)                      cgAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGc                                                <  2:673863/76‑1 (MQ=255)                       gAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCa                                               >  1:715030/1‑76 (MQ=255)                           gTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTg                                           <  2:1139068/76‑1 (MQ=255)                            tCTTCCTGACCGCGAGACAATANTAATCATTCTCATTCNCACTTGTTCAACAGTTTTAGCAAAACAATGCNATTGa                                          >  1:24415/1‑76 (MQ=255)                              ttCNTGTTCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTgccc                                        <  2:1309036/76‑4 (MQ=255)                              ttCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACt                                        >  1:628977/1‑76 (MQ=255)                              ntCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACt                                        >  1:509365/2‑76 (MQ=255)                               tCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTc                                       <  2:631526/76‑1 (MQ=255)                               tCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTc                                       >  2:784336/1‑76 (MQ=255)                               tCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTc                                       <  1:517899/76‑1 (MQ=255)                               tCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACANNTTCAACAATTNNTGCAAAAAAATGCAGTTGACTc                                       >  1:1301923/1‑76 (MQ=255)                                  tGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCa                                    <  2:1273268/76‑1 (MQ=255)                                  tGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTNNAACACTTTTTNNAGAAAAATGCATTTGACTCGCa                                    >  1:1297545/1‑76 (MQ=255)                                   gATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCAt                                   >  2:280201/1‑76 (MQ=255)                                   gATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCAt                                   >  1:805416/1‑76 (MQ=255)                                   gATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCAt                                   >  2:568809/1‑76 (MQ=255)                                          agaCAATAATAATCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAg                            >  1:1019155/1‑76 (MQ=255)                                              aataataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCaa                        >  1:1188638/1‑76 (MQ=255)                                              aataataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCaa                        <  1:468967/76‑1 (MQ=255)                                               ataataatCATTCTCATTCNCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAat                       >  1:1343531/1‑76 (MQ=255)                                               ataataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAat                       <  1:500577/76‑1 (MQ=255)                                               ataataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAat                       >  1:446230/1‑76 (MQ=255)                                                taataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAatg                      >  2:1246313/1‑76 (MQ=255)                                                taataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAatg                      >  1:998510/1‑76 (MQ=255)                                                taataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAatg                      >  2:520543/1‑76 (MQ=255)                                                 aataatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAatga                     <  1:991816/76‑1 (MQ=255)                                                    aatCATTCTCATTCGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGt                  >  1:765781/1‑76 (MQ=255)                                                      tcattctcattcGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTg                <  2:944708/76‑1 (MQ=255)                                                             cattcGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGa         <  2:977118/76‑1 (MQ=255)                                                             cattcGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGa         <  1:1162776/76‑1 (MQ=255)                                                               ttcGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACa       <  1:1134542/76‑1 (MQ=255)                                                                tcGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACAt      >  2:987855/1‑76 (MQ=255)                                                                 cGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACAtt     <  1:425579/76‑1 (MQ=255)                                                                 cGCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACAtt     >  1:1278029/1‑76 (MQ=255)                                                                  gCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACAttt    <  2:1424645/76‑1 (MQ=255)                                                                  gCACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACAttt    <  1:694852/76‑1 (MQ=255)                                                                   cACTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTa   >  2:827647/1‑76 (MQ=255)                                                                    aCTTGTTCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTAc  >  1:611515/1‑76 (MQ=255)                                                                          |                                                                      GTCGTGCTTTCAGGTTCTCCGCGAGGGTCTTCCTGATCGCGAGACAATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTAC  >  REL606/3512430‑3512572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑