Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,995,676	(C)8→7	intergenic (+103/+351)	yeeN → / ← asnW	hypothetical protein/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,995,669	0	C	.	100.0%	127.9 / NA	26	intergenic (+96/+358)	yeeN/asnW	hypothetical protein/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (19/7);  total (19/7)

TTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCA  >  REL606/1995597‑1995737                                                                         |                                                                     ttACGGCATATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTTATTATAcccc                                                                   <  1:133909/76‑1 (MQ=255)   aCGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccc                                                                 >  2:548756/1‑76 (MQ=255)          aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc                                                         <  1:384513/76‑1 (MQ=255)            ttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCag                                                       >  2:144696/1‑76 (MQ=255)             tCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCaga                                                      >  1:290509/1‑76 (MQ=255)                 tAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGaaa                                                  >  2:511312/1‑76 (MQ=255)                    tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaataa                                               <  2:715152/76‑1 (MQ=255)                     ctgatatAATTCTTATAAAAAATATANTATTCATCTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaataat                                              >  2:834788/4‑76 (MQ=255)                      tcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAataata                                             >  2:705159/1‑76 (MQ=255)                       cATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAatat                                            >  1:424183/1‑76 (MQ=255)                                 ttATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAAt                                  >  2:114656/1‑76 (MQ=255)                                   ataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTc                                <  1:323329/76‑1 (MQ=255)                                             taatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                      <  1:372247/76‑1 (MQ=255)                                             taatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                      >  2:689315/1‑76 (MQ=255)                                                tatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCtttt                   >  1:316991/1‑76 (MQ=255)                                                 atTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                  >  2:769431/1‑76 (MQ=255)                                                   tCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTTTTCttttttt                >  1:282173/1‑74 (MQ=255)                                                   tCAACTCGTCATATTGATCATA‑CCCCCCCGTTCCCAGAGAAATAATACATATTAAAATTCCAGTTCTTCTTTTTCt                >  2:872000/1‑76 (MQ=255)                                                     aaCTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa              >  1:919942/1‑76 (MQ=255)                                                     aaCTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGa              >  1:394296/1‑76 (MQ=255)                                                      aatCGTCCTATTGATTATA‑CCCCCCCGTTCCCCNAGACATCATCTTTATTAAAATTCNAGTTCTTCTTTTTCTGAt             >  2:101137/3‑76 (MQ=255)                                                        tCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa           >  1:314777/1‑76 (MQ=255)                                                         cGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc          >  1:771478/1‑76 (MQ=255)                                                         cGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc          <  1:348897/76‑1 (MQ=255)                                                          gTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa         >  1:559988/1‑76 (MQ=255)                                                              tatTGATTATA‑CCCCCCCGTTCTCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg     <  2:919941/76‑1 (MQ=255)                                                              tatTGATTATA‑CCCCCCCGTTCCCAGCGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg     >  1:852952/1‑76 (MQ=255)                                                              tatTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg     <  2:394296/76‑1 (MQ=255)                                                                 tGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAANTTCCAGATCTTCTTTTTCTGATTACAGAAGGCa  >  1:778438/1‑76 (MQ=255)                                                                 tGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCa  >  1:408354/1‑76 (MQ=255)                                                                         |                                                                     TTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCA  >  REL606/1995597‑1995737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑