Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	2,448,493	IS186 (–) +6 bp	intergenic (+24/+21)	nupC → / ← yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2448493 =	1 (0.040)	36 (1.270)	27/144	0.2	97.1%	intergenic (+24/+26)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	2772854 =	NA (NA)					noncoding (1/1343 nt)	IS186	repeat region
*	?	REL606	= 2448498	0 (0.000)	32 (1.130)	26/144	0.2	100%	intergenic (+29/+21)	nupC/yfeA	nucleoside (except guanosine) transporter/predicted diguanylate cyclase
	?	REL606	= 2774196	NA (NA)					noncoding (1343/1343 nt)	IS186	repeat region

ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448565‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  >  REL606/2772854‑2772931                                                                                                                                                         ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCC                                                                             >  2:488382/1‑76      CAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAG                                                                        >  1:26019/1‑76       AAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGC                                                                       >  1:280136/1‑76            TATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAA                                                                  >  2:493412/1‑76               TTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTT                                                               >  1:47887/1‑76               TTGATTGGTCGCCCGCAGACATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTT                                                               <  1:733339/76‑1                 GATTGGCCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAA                                                             >  1:146171/1‑76                  ATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGTGAATTATCCCATAAGCGCTAACTTAAG                                                            <  1:89529/76‑1                  ATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAG                                                            <  1:147898/76‑1                     GGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGT                                                         <  2:96270/76‑1                        CGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGT                                                      >  2:920660/1‑76                        CGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGT                                                      <  2:919356/76‑1                          CCCGCAGCTATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTACCTTAAGGGTTGTGG                                                    >  2:633207/1‑76                           CCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGT                                                   >  1:90290/1‑76                            CGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCGATAAGCGCTAACTTAAGGGTTGTGGTA                                                  >  1:559770/1‑76                               AGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTA                                               <  2:507273/76‑1                                GCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAC                                              <  1:483417/76‑1                                GCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAC                                              <  1:68429/76‑1                                  CATTAGCTGATTCACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                            >  2:153752/1‑76                                  CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                            >  1:651488/1‑76                                  CATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                            >  2:230409/1‑76                                    TTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                          <  2:556953/76‑1                                     TAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTG                                         >  1:578363/1‑76                                              AACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                >  1:342729/1‑76                                               ACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTA                               <  1:382117/76‑1                                               ACAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTA                               <  1:294286/76‑1                                                CAGGCGTAAAAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAA                              <  2:47887/76‑1                                                         AAAAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATAATTTAACGTGCCGAT                     <  2:609047/76‑1                                                           AAACCGGGGAATTATCCCNTAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAGGTGCCGATGA                   <  1:758615/76‑1                                                           AAACCGGGGAATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGA                   <  2:741057/76‑1                                                             ACCGGGGAATTATCCCATAAGCGCTAACTGAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAT                 <  2:90290/76‑1                                                                    AATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTC          <  1:28606/76‑1                                                                     nTTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTGTCA         <  1:837457/75‑1                                                                     ATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA         <  1:681565/76‑1                                                                     ATTATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA         >  1:907116/1‑76                                                                        aaCCCATAAGCGCTAACTTAAGGGTTGTTGTATTACGCCTGATATGATTTAACGTGCTGATGAATTACTCTCACGA      <  2:873443/74‑1                                                                        ATCCCATAAGCGCGAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGA      <  2:49810/76‑1                                                                         TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCATGATATGATTTAACGTGCCGATGAATTACTCTCACGAT     <  1:685479/76‑1                                                                          CCCATAAGCGCTAACTTAATGGTTGTGGTATTACGCCTGATATGATTTGACGTGCCGATGAATTACTCTCACGATA    <  1:94729/76‑1                                                                          CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA    <  2:943641/76‑1                                                                          CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATA    <  1:168653/76‑1                                                                           CCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAA   <  1:485685/76‑1                                                                            CATAAGTGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  <  1:869770/76‑1                                                                            CATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  >  1:368955/1‑76                                                                                                                                                         ATCTGCAAGGGTATTTGATTGGTCGCCCGCAGCCATTAGCTGATTAACAGGCGTAAAAAAACCGGGGAATTATccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2448565‑2448493 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC  >  REL606/2772854‑2772931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.