| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,396,510 | 1 | . | G | 20.0% | 51.8 / 15.1 | 20 | coding (99/756 nt) | yjfQ | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base . (2/14); new base G (4/0); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCAGGAGGATT > REL606/4396436‑4396585 | gCTTCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑g < 2:61053/76‑1 (MQ=255) cTTCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑gg > 2:945688/1‑76 (MQ=255) ttCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑ggg > 1:941366/1‑75 (MQ=255) ttCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGc < 1:329996/76‑1 (MQ=255) tCTGCGCCATTTCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCg < 2:35911/76‑1 (MQ=255) cATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGGGGAAATcc > 2:703924/1‑76 (MQ=255) cgcACTTTTTTCTGTTTACTGCTGNCGTCAAGTTTATTGATATCGCGTCGCGCAGCGGNA‑GGCGAAATTCCCAGAc < 2:801226/76‑1 (MQ=255) cTTTTTCCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGGAAGTTCCCGACACTc > 1:144562/1‑76 (MQ=255) ttttCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGGGGAAATACCCGACGCCCaaa > 1:384082/1‑75 (MQ=255) ttttCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAAc < 2:333507/76‑1 (MQ=255) aGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCANTGGCA‑GGGGGAAATCCCAGACGCCCCACGACtt > 1:773150/1‑76 (MQ=255) aGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACtt < 1:380614/76‑1 (MQ=255) aGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCACGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACtt < 1:685616/76‑1 (MQ=255) cGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGAAATGCCCAGACGCTCAACGACTTTCTCAAc < 1:853094/76‑1 (MQ=255) tttCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCAGGGGGAAATTCCC‑GACGCCCCACGACCTTTTCCAcagg > 2:108329/1‑73 (MQ=255) ttCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCAGGGGGAAATTCCC‑GACGCCCCACCACTTTTTCAACCGTc > 1:532447/1‑76 (MQ=255) ttGATATGGCGTCGCGCAGTGGCAGGGGGAAATTCCC‑CACGCTCAACGACCTTTTCCACCGCCACAAAACCCaaat > 1:622650/1‑74 (MQ=255) tGATATCGCGTCGCGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATtg < 1:203881/76‑1 (MQ=255) atatCGCGTCGCGCAGTGNCA‑GGCGAAATTCCCTGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATtgtg < 1:758646/76‑1 (MQ=255) cgcgtcgcgCAGTGGCAGGGGGAAATTCCC‑GACGCTCCACGACCTTTTCCACCGTCCCCAAAACCAACTTTGCggg > 1:717653/1‑74 (MQ=255) cgtcgcgCAGTGGCA‑GGCGAAATGCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCa < 2:300960/76‑1 (MQ=255) cgcAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTcc < 2:541968/76‑1 (MQ=255) gTGGCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCagga < 2:927419/76‑1 (MQ=255) ggCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCaggagg < 1:959788/76‑1 (MQ=255) cA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCAGGAGGAt < 2:596728/76‑1 (MQ=255) a‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCAGGAGGAtt > 2:107092/1‑76 (MQ=255) | GCTTCTGCGCCATTGCGCACTTTTTTCAGTTTGCCGCTTTCGTCAAGTTTATTGATATCGCGTCGCGCAGTGGCA‑GGCGAAATTCCCAGACGCTCAACGACTTTCTCAACGGTCACAAAGCCCAATTGTGCGAGCATTTCCAGGAGGATT > REL606/4396436‑4396585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |