Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	649,601	T→C	I401V (ATC→GTC)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	649,601	0	T	C	100.0%	65.0 / NA	17	I401V (ATC→GTC)	mrdA	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

TCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649534‑649673                                                                    |                                                                         tgcGCCACGTCGATACCGGTGTAATGACCTTATCCGAATTTACCCATCAACTCGGTGAGGCGATCGACCCCCatat                                                                  <  1:106119/74‑1 (MQ=255) tcCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCatat                                                                  >  2:125077/1‑76 (MQ=255)      cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGGTCGACCCCCa                                                                     <  1:473364/68‑1 (MQ=255)      cAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCa                                                                     >  2:473364/1‑68 (MQ=255)        ggTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACACCCATATCGAAGGc                                                           >  2:595515/1‑76 (MQ=255)             aTACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCt                                                      <  2:378053/76‑1 (MQ=255)              tACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTg                                                     >  2:357219/1‑76 (MQ=255)               aCCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGa                                                    >  1:262779/1‑76 (MQ=255)                   gtgtAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCACCATATCGTAGGCCACCTGATaga                                                >  1:297576/1‑76 (MQ=255)                    tgtAACGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATagaa                                               <  2:753795/76‑1 (MQ=255)                       aaTGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAgaagaa                                            >  1:251062/1‑76 (MQ=255)                            ccATAACCGAACTTATCTATCCATTCGGTGAGGCGCTCGACCNCNATATCGTNGGCCACCTGATAGAAGAAGGTAt                                       <  1:891070/76‑1 (MQ=255)                                    gAATTTACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAt                               <  2:563841/76‑1 (MQ=255)                                       tttACCCATCCATTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGAttct                            >  1:498815/1‑76 (MQ=255)                                                  atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 >  2:383839/1‑76 (MQ=255)                                                  atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 >  2:675793/1‑76 (MQ=255)                                                  atTCGGAGAGGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCt                 >  2:95501/1‑76 (MQ=255)                                                      ggAGAGGCGATCGACCCCCATATCGTAGGCCACATGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTtgtg             <  1:765823/76‑1 (MQ=255)                                                          tggCGACCGATCCCCATATCGGAGGCCACCTGATAGAAGAAGGTATCCGGAGATTCTTCCACCGATCTGGTGACag         >  2:640449/2‑75 (MQ=255)                                                          agGCGATCGACCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACAt         >  2:416186/1‑76 (MQ=255)                                                              gatcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATTCGCAGATTCTTCCAGCCATCTTGTGACATTCAg     >  2:643576/1‑76 (MQ=255)                                                                tcgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGAc   <  2:45135/76‑1 (MQ=255)                                                                 cgaccCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACg  >  2:16955/1‑76 (MQ=255)                                                                    |                                                                         TCCGCCAGGTCGATACCGGTGTAATGACCATAACCGAATTTACCCATCCATTCGGAGAGGCGATCGATCCCCATATCGTAGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACG  >  REL606/649534‑649673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑