Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	74.0 / NA	27	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/14);  total (13/14)

GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193709‑2193850                                                                       |                                                                        gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                    >  2:189573/1‑76 (MQ=255)       tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                              >  2:326963/1‑76 (MQ=255)        ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                             >  1:166179/1‑76 (MQ=255)        ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                             <  1:264285/76‑1 (MQ=255)          ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           <  2:907424/76‑1 (MQ=255)             gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        <  1:214974/76‑1 (MQ=255)                 aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                    >  2:525103/1‑76 (MQ=255)                                ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:511505/76‑1 (MQ=255)                                gacTGTCGGAAATCAGCCCCAAGAANAGCAAACCGCTAATCCCGACCAGATANAGCATGACGNCTGTCATANACan                                     >  1:827275/3‑75 (MQ=255)                                    gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAAATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:85801/76‑1 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATNAAGCATAACGCCTGTATTAAACa                                      >  1:775806/1‑66 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      <  2:274249/66‑1 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      <  2:775806/66‑1 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:274249/1‑66 (MQ=255)                                         aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      >  1:626021/1‑66 (MQ=255)                                         aaaTCAACCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                      <  2:626021/66‑1 (MQ=255)                                           aTCCGCCCCAATANCAGCAANACGCCTATCCCCACCNGATAAAGCACGACGNCTGTATTAAANAGGCTTTTTACCg                          <  2:827987/76‑1 (MQ=255)                                           aTCAGCCCCAATAAGAGAAAAACGACAATCGACACAAGATAANGNATGACACNTGTATTAAACAGGCTTCTTACCg                          <  1:892728/76‑1 (MQ=255)                                              aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAAAGGCTTTTTACCGATg                       >  2:646023/1‑76 (MQ=255)                                                 cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                    >  2:194377/1‑76 (MQ=255)                                                 cccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                    <  1:581820/76‑1 (MQ=255)                                                     aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                <  1:189573/76‑1 (MQ=255)                                                             aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  2:189127/1‑76 (MQ=255)                                                             aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        <  1:603001/76‑1 (MQ=255)                                                                 cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg    <  2:30320/76‑1 (MQ=255)                                                                 cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg    >  2:72673/1‑76 (MQ=255)                                                                 cGCCAATCCCCAACGGATAGAGCTNGACGCCTGTATTAAACAGGCTTTTTACCGAAGGNGCATCGCGCACCAgccg    <  2:796474/76‑1 (MQ=255)                                                                   ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  >  1:345091/1‑76 (MQ=255)                                                                       |                                                                        GATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193709‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑