| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,194,694 | Δ1 bp | intergenic (+40/‑229) | metA → / → aceB | homoserine O‑succinyltransferase/malate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,194,694 | 0 | A | . | 96.5% | 131.9 / ‑4.0 | 29 | intergenic (+40/‑229) | metA/aceB | homoserine O‑succinyltransferase/malate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (5/23); minor base G (0/1); total (5/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCAT > REL606/4194624‑4194763 | tCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAg < 2:1120034/76‑1 (MQ=255) cGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑c < 2:836940/76‑1 (MQ=255) cacaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCtt < 2:596012/76‑1 (MQ=255) caTGAATCCAACGCTGGATTAATCTTCTGTGTTAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCg < 2:598788/76‑1 (MQ=255) tGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCggg < 1:988062/76‑1 (MQ=255) atgCTGGATTAAGCTGTTGTGGTAGGCGATCGTTAAGCCATTCAGCCCCTT‑CCTCAGGCA‑CCTTCGGGTGC‑Ctttt < 2:943792/74‑1 (MQ=255) aCGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑Ctttt < 2:943824/76‑1 (MQ=255) gCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑Ctttttt < 1:1050306/76‑1 (MQ=255) gCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑Ctttttt < 2:87364/76‑1 (MQ=255) gCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑Ctttttt < 1:1050291/76‑1 (MQ=255) tGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTAt < 2:660577/76‑1 (MQ=255) gATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTAttt < 2:88445/76‑1 (MQ=255) gATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTAttt < 2:31826/76‑1 (MQ=255) gATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTAttt < 2:568054/76‑1 (MQ=255) aTTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTc < 1:981241/76‑1 (MQ=255) aaTCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCCCGCACCCTCCGGGGGCGCtttttttttttt > 1:243474/1‑74 (MQ=255) aTCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGaa < 2:382222/76‑1 (MQ=255) ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTa < 2:666499/76‑1 (MQ=255) tCGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTcagcag < 2:70044/76‑1 (MQ=255) ggATCGTTAGGCGATTCAGCGCCTT‑CCTCAGGCA‑ACTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAgg < 1:341654/75‑1 (MQ=255) cGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAgg < 1:341638/76‑1 (MQ=255) cGATCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGGGC‑CTTATTTTTTTTCGAAACGTACCTCAGCAgg > 1:49510/1‑76 (MQ=255) aTCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTg < 2:296436/76‑1 (MQ=255) aTCGTTAAGCGATTCAGCACC‑TGTCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTg < 2:274041/76‑1 (MQ=255) tCGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGa < 1:916181/76‑1 (MQ=255) cGTTAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTNCGGGGGC‑CTTTTTTATTTTCGANACGTTCCTGCNCAGGTGaa > 2:1015324/1‑76 (MQ=255) tAAGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATaa < 1:184034/76‑1 (MQ=255) aGCGATTCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTTTTTTCCAAAACTACCCCAGCAGGGGAATAAAt > 2:825467/1‑76 (MQ=255) ttCAGCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTAt < 2:606265/76‑1 (MQ=255) gCACCTT‑CCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCat > 1:207509/1‑76 (MQ=255) | TCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCA‑CCTTCGGGTGC‑CTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCAT > REL606/4194624‑4194763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |