| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 30,364 | G→C | A6P (GCC→CCC) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 30,364 | 0 | G | C | 97.6% | 160.1 / NA | 42 | A6P (GCC→CCC) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (20/21); minor base T (0/1); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GACCGTTCATTTTGATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGGCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTAC > REL606/30293‑30435 | gACCGTTCATTTTGATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGTTCCTGTTGCCCaa < 1:77444/76‑1 (MQ=255)gACCGTTCATTTTGATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCaa < 1:190366/76‑1 (MQ=255) ttCATTTTGATATTGAAGTGCTGGAAACCGATCCGGCACTGGAGNCGNAACATGCAGATCCTNTTGCCCAACCcgc > 2:1085138/1‑76 (MQ=255) cATTTTGATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGt > 1:291140/1‑76 (MQ=255) gATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGtttt < 1:1031688/76‑1 (MQ=255) gATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGtttt < 1:776860/76‑1 (MQ=255) gATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATACTGTTGCCCANCCCGCGTGGtgtg > 1:152083/1‑73 (MQ=255) tatTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTtg > 2:519681/1‑76 (MQ=255) gCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGt < 1:596304/76‑1 (MQ=255) gCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTNTTGTGCCGGGGt < 1:11297/76‑1 (MQ=255) tGGAAATCNAGCCGGCACTNGAGGCGTAACATGCAGATCCTGNTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAg < 2:1008266/76‑1 (MQ=255) tGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAg < 1:708191/76‑1 (MQ=255) ggAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGa > 1:307737/1‑76 (MQ=255) aaaTCGATCCGGCACTGGAGGCGTAACATGCAGNTCCTGTTGCCCAACCCGCGTGGNTTTTGTGCAGNGGTAGAcc > 2:1018041/1‑76 (MQ=255) ccGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATc > 1:234629/1‑76 (MQ=255) ccGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATc < 1:928654/76‑1 (MQ=255) cGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGAGGTAGACCGCGCTATCa > 1:136920/1‑76 (MQ=255) cGGCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGCGGCGGGGTAGACCGCGCTATCa < 1:6069/76‑1 (MQ=255) gCACTGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGc < 1:187969/76‑1 (MQ=255) aCTNGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGNTTTTGTGCCGGGGTAGACCGCGCTATCAGCAt < 2:1133926/76‑1 (MQ=255) aCTGGAGGCGTAACATGCAGATCCTGTTGCCCCACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTCTCAGCAt > 2:546719/1‑76 (MQ=255) tGGAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCAttg > 2:850345/1‑76 (MQ=255) ggAGGCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCAttgt > 1:855936/1‑76 (MQ=255) ggCGTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGa < 2:833256/76‑1 (MQ=255) gTAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGAACGCGCTATCCGCATTGTTGaaaa > 1:1039274/1‑76 (MQ=255) tAACATGCAGATCCTGTTGCCCGACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAc > 1:310666/1‑76 (MQ=255) tAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAc > 2:465195/1‑76 (MQ=255) tAACATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAc > 2:839646/1‑76 (MQ=255) aaCATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAcg < 1:844759/76‑1 (MQ=255) aaCATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAcg < 1:786242/76‑1 (MQ=255) cATGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAAcgcg > 1:267206/1‑76 (MQ=255) tGCAGATCCTGTTGTCCAACCCGCCTGGGTTNTGTGCCGGTGTAGANCGCGCNATCAGCATTGTTGTAAACGCGCt < 2:151578/76‑1 (MQ=255) tGCAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCt > 1:682932/1‑76 (MQ=255) cAGATCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTgg > 2:398140/1‑76 (MQ=255) tCCTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCAt < 2:11238/76‑1 (MQ=255) ccTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCAtt > 2:122529/1‑76 (MQ=255) ccTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCAtt < 2:359530/76‑1 (MQ=255) cTGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCAttt < 2:421977/76‑1 (MQ=255) tGTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTa < 1:805944/76‑1 (MQ=255) gtttGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTa < 1:969223/74‑1 (MQ=255) gTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTAc > 1:163888/1‑76 (MQ=255) gTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTAc < 2:749039/76‑1 (MQ=255) gTTGCCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTAc < 1:943678/76‑1 (MQ=255) | GACCGTTCATTTTGATATTGAAGTGCTGGAAATCGATCCGGCACTGGAGGCGTAACATGCAGATCCTGTTGGCCAACCCGCGTGGTTTTTGTGCCGGGGTAGACCGCGCTATCAGCATTGTTGAAAACGCGCTGGCCATTTAC > REL606/30293‑30435 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |