Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,465,213	A→C	L19R (CTG→CGG)	ompR ←	osmolarity response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,465,213	0	A	C	100.0%	168.4 / NA	40	L19R (CTG→CGG)	ompR	osmolarity response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/13);  total (27/13)

AGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCAAA  >  REL606/3465141‑3465284                                                                         |                                                                        agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCcgcg                                                                      <  1:1016784/76‑1 (MQ=255) agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCcgcg                                                                      >  2:500535/1‑76 (MQ=255)    cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac                                                                   >  2:873742/1‑76 (MQ=255)    cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac                                                                   >  2:632261/1‑76 (MQ=255)    cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac                                                                   <  2:686485/76‑1 (MQ=255)     gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcacg                                                                  >  1:489394/1‑76 (MQ=255)        ccATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAg                                                               >  2:525405/1‑76 (MQ=255)        ccATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAg                                                               <  1:386298/76‑1 (MQ=255)          aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATATCCTTCCAGCCGCGCACGCAGgc                                                             <  1:8332/76‑1 (MQ=255)           tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcg                                                            >  2:804168/1‑76 (MQ=255)            cTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcgc                                                           >  1:949751/1‑76 (MQ=255)            cTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCGTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcgc                                                           <  1:170401/76‑1 (MQ=255)                  tGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATgtcg                                                     >  2:31698/1‑76 (MQ=255)                    cATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATgtcgtc                                                   >  2:726939/1‑76 (MQ=255)                     aTTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCa                                                  <  2:614880/76‑1 (MQ=255)                        aGCGACGCTTCGAACCTGGAAGCCTTGTTCGCTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCg                                               <  2:198635/76‑1 (MQ=255)                           gACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGacc                                            >  2:869315/1‑76 (MQ=255)                               cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca                                        <  1:607027/76‑1 (MQ=255)                               cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca                                        >  1:499875/1‑76 (MQ=255)                               cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca                                        >  2:465810/1‑76 (MQ=255)                                ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg                                       >  1:941829/1‑76 (MQ=255)                                ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg                                       >  1:401908/1‑76 (MQ=255)                                ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg                                       >  2:458879/1‑76 (MQ=255)                                   gAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAat                                    <  2:544343/76‑1 (MQ=255)                                       ttGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg                                >  1:797021/2‑76 (MQ=255)                                       cTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg                                >  1:797035/1‑76 (MQ=255)                                       cTGGAAGCCTTGTTCGGAGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg                                >  2:84682/1‑76 (MQ=255)                                        tGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt                               >  2:224533/1‑76 (MQ=255)                                          gAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg                             <  1:449931/76‑1 (MQ=255)                                              ccTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGNCATCGACCACCAGAATATTGTAGTtct                         <  1:1208031/76‑1 (MQ=255)                                                 tGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCtt                      <  1:191571/76‑1 (MQ=255)                                                 tGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCtt                      <  1:520889/76‑1 (MQ=255)                                                  gTTCGGTGAGATAACGTTCCAGCCGCGCACCCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTCTTCTTg                     >  1:1100993/1‑76 (MQ=255)                                                   ttCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGc                    >  1:376839/1‑76 (MQ=255)                                                       gTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCAttg                >  2:225440/1‑76 (MQ=255)                                                        tGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCAttgt               >  2:608382/1‑76 (MQ=255)                                                          agaTAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCAACGACCACCAGAATATTGTAGTTCTCTTGCAttgttt             <  2:203143/76‑1 (MQ=255)                                                              aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTNATCGACCACCAGAATATTGTAGNTCTCTTGCCTNGTTTGTAc         >  2:1011022/1‑76 (MQ=255)                                                              aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTNATCGACCACCAGAATATTGTAGNTCTCTTGCCTNGTTTGTAc         >  2:1011008/1‑76 (MQ=255)                                                              aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTAc         >  1:735762/1‑76 (MQ=255)                                                                 gTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTcc      >  1:312283/1‑76 (MQ=255)                                                                    ccAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaa   >  2:758680/1‑76 (MQ=255)                                                                     cagccgCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaaa  >  2:713960/1‑76 (MQ=255)                                                                     cagccgCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaaa  >  2:992687/1‑76 (MQ=255)                                                                         |                                                                        AGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCAAA  >  REL606/3465141‑3465284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑