Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,498,010	G→A	S9L (TCG→TTG)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,498,010	0	G	A	100.0%	132.1 / NA	34	S9L (TCG→TTG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/24);  total (10/24)

GGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTC  >  REL606/3497938‑3498077                                                                         |                                                                    gggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATg                                                                  <  1:886991/76‑1 (MQ=255)  ggTCCTTTCCAATCGTATACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGa                                                                 <  1:128617/76‑1 (MQ=255)     ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAata                                                              >  2:963842/1‑76 (MQ=255)     ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAata                                                              >  1:348343/1‑76 (MQ=255)      cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAatat                                                             <  1:1161554/76‑1 (MQ=255)      cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAatat                                                             <  2:132032/76‑1 (MQ=255)        ttCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGt                                                           >  2:1162261/1‑76 (MQ=255)           cAATCAAAAGCATGNGCTTGTAACGGATAGAGTGCTTAAGCGCTCATACGCTAAGCGTGGGCGATGAATATGTaan                                                        <  2:171007/76‑2 (MQ=255)            aaTCGTAAGCATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAAc                                                       <  1:1082772/76‑1 (MQ=255)               cGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGa                                                    <  2:733791/76‑1 (MQ=255)                gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAg                                                   >  2:560243/1‑76 (MQ=255)                   aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCAt                                                <  1:174713/76‑1 (MQ=255)                          gCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCAtata                                         <  1:618432/76‑1 (MQ=255)                           cTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAg                                        <  2:517832/76‑1 (MQ=255)                           cTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAg                                        >  1:974734/1‑76 (MQ=255)                                aaGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGtt                                   >  2:1161008/1‑76 (MQ=255)                                 aGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGttt                                  <  2:858135/76‑1 (MQ=255)                                   cGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTcc                                >  1:552397/1‑76 (MQ=255)                                     aTAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTg                              >  2:894661/1‑76 (MQ=255)                                      tAGAGTGCTTAAGNGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGa                             <  2:1110278/76‑1 (MQ=255)                                       agagTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaa                            >  1:558070/1‑76 (MQ=255)                                            gCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATGGGCGTTTCCTGAAAACTa                       <  1:90134/76‑1 (MQ=255)                                               tAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTAttt                    <  2:1867/76‑1 (MQ=255)                                                aaGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                   <  1:1114194/76‑1 (MQ=255)                                                 aGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                  <  2:123994/76‑1 (MQ=255)                                                 aGGGCTTCTACGCNAAGCGTGGGCAATGAATATGTAAACGGAGCAATCATATAGGCGTTTCCTGAAAACTATTTCa                  <  2:1107883/76‑1 (MQ=255)                                                  gggCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCaa                 <  2:481956/76‑1 (MQ=255)                                                   ggCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                <  2:350591/76‑1 (MQ=255)                                                        cTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa           <  1:963843/76‑1 (MQ=255)                                                          aCGNTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATNGGCGTTTCTTGAAAACTATTTCAAGCGATAGt         <  2:1128416/76‑1 (MQ=255)                                                               aaGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGc    >  1:659218/1‑76 (MQ=255)                                                                aGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt   <  2:372501/76‑1 (MQ=255)                                                                 gCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATATTAAAGCTc  <  2:1170334/76‑1 (MQ=255)                                                                 gCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTc  <  2:1170336/76‑1 (MQ=255)                                                                 gCGTGGGCAATGAAGATGTAATCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTc  <  2:181296/76‑1 (MQ=255)                                                                         |                                                                    GGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTC  >  REL606/3497938‑3498077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑