| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,162,513 | C→T | T553I (ACT→ATT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,162,513 | 0 | C | T | 97.1% | 122.9 / ‑3.9 | 34 | T553I (ACT→ATT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/15); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
TATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTC > REL606/4162442‑4162582 | tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCAc > 2:764660/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTAcag > 2:519148/1‑74 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 2:589501/1‑76 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 1:829862/1‑76 (MQ=255) cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGATTCACTACGGt > 2:472823/1‑76 (MQ=255) gCACTCGGCCCAGGCGGCCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTc < 1:127820/76‑1 (MQ=255) tCGGCCCAGGCGGCCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGt > 1:81773/1‑76 (MQ=255) cGGCCCAGGCGGTCTGACCCGTGAACGCGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCCCTACGGTCGCGTa > 2:784031/1‑76 (MQ=255) ggCCCAGGCGGTCTGACCCGTGAACGTGCAAGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTAGCGTAt > 1:1105626/1‑76 (MQ=255) ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTc < 1:559450/76‑1 (MQ=255) aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCa < 1:998680/76‑1 (MQ=255) ggCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCaa > 2:257478/1‑76 (MQ=255) gCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAAt < 1:674629/76‑1 (MQ=255) cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcccc > 1:801423/1‑76 (MQ=255) cGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTg < 2:683634/76‑1 (MQ=255) gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 2:635902/76‑1 (MQ=255) gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 1:221151/76‑1 (MQ=255) tGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg < 2:1182492/76‑1 (MQ=255) aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc > 2:1059422/1‑76 (MQ=255) ggCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACa < 1:89505/76‑1 (MQ=255) gCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt < 1:891453/76‑1 (MQ=255) cTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc > 2:579542/1‑76 (MQ=255) tCGAAGTTCGCGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCgg > 1:1120361/1‑76 (MQ=255) cGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt > 1:694155/1‑76 (MQ=255) gAAGTTCGACACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc > 2:1041338/1‑76 (MQ=255) aGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 2:754567/76‑1 (MQ=255) gTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa < 1:909734/76‑1 (MQ=255) cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 2:648551/1‑76 (MQ=255) gagaCTTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa > 1:1172598/1‑76 (MQ=255) aCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc < 1:1074276/76‑1 (MQ=255) cGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTNATCAActct < 1:30131/76‑1 (MQ=255) tACACCCGATTCACTACGGTCGCGTATGTGCAATCGTAACCCCTGAAGGTCCGAACANCGGTCTGATCAActctct < 1:1154489/76‑1 (MQ=255) acacCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg < 2:449955/76‑1 (MQ=255) acacCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg > 1:811736/1‑76 (MQ=255) acCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc > 2:713734/1‑76 (MQ=255) | TATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTC > REL606/4162442‑4162582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |