Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,184,059	G→T	A385A (GCG→GCT)	zraR →	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,184,059	0	G	T	100.0%	120.6 / NA	34	A385A (GCG→GCT)	zraR	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/17);  total (17/17)

GAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTG  >  REL606/4183988‑4184131                                                                        |                                                                         gAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTg                                                                      <  2:1192534/76‑1 (MQ=255)    aaggcgGTGGAAGGGGCAGGGGTGCGGCTGAGGGGGGAATATATCTCCGAACGCGAGCTGCCGCTGGCTATTGCCa                                                                   <  2:791276/73‑1 (MQ=255)    aaCGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCa                                                                   <  2:791293/76‑1 (MQ=255)        cgGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTAc                                                               >  1:482617/1‑76 (MQ=255)           tGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGcc                                                            <  1:649930/76‑1 (MQ=255)             gAACTGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGa                                                          <  2:1205793/76‑1 (MQ=255)             gAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGa                                                          >  1:324413/1‑76 (MQ=255)                cGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATcc                                                       <  1:319979/76‑1 (MQ=255)                 gggCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATccc                                                      <  2:324288/76‑1 (MQ=255)                          tgctgcTGACCGGGAAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACa                                             <  1:662682/76‑1 (MQ=255)                           gctgctGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACaa                                            >  2:1079139/1‑76 (MQ=255)                           gctgctGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTCGTACaa                                            <  1:74041/76‑1 (MQ=255)                             tgctgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAg                                          <  2:893712/76‑1 (MQ=255)                                tgACCGGGGTATATATTTCCGAANGCGAGNTGCCGATGGCTATTNCCTGTAGGCCGATCCCACTGGGACAAAGTCa                                       >  2:152784/1‑76 (MQ=255)                                tgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCa                                       >  1:489565/1‑76 (MQ=255)                                 gACCGGGGAATATATTTCCGAAGGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAg                                      >  1:5101/1‑76 (MQ=255)                                   ccGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGa                                    >  2:480716/1‑76 (MQ=255)                                      gggAATATNTGCCCGAACGNGGGCTGCCGCTGGCTATTGCCANTACGCCGGTCCCGCTGGGACAAAGTCAGGatat                                 <  2:1019389/76‑1 (MQ=255)                                       ggAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATAtt                                <  1:176762/76‑1 (MQ=255)                                         aaTATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCa                              >  2:523211/1‑76 (MQ=255)                                         aaTATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCa                              >  1:750993/1‑76 (MQ=255)                                           tatatTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGc                            >  2:961871/1‑76 (MQ=255)                                            atatTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCACGCCGGGGCAAAGCGAGGATTTTAAgac                           >  2:1076845/1‑74 (MQ=255)                                                ttCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCNGCNGGGACAAAGTCAGGNTATTCAGCCGTtg                       >  2:1087693/1‑76 (MQ=255)                                                tcccGACCGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTtg                       <  2:638019/74‑1 (MQ=255)                                                     aaCGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGAGCCCGCTGGGACAAAGTCAGGNTATTCAGCCGTTGgtgga                  <  1:1155718/76‑1 (MQ=255)                                                       ggcgAGCTGCCGCTGGATGTTGTCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                <  2:46234/75‑1 (MQ=255)                                                       cgggAGCTGCCGCTGGCTATTGACAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                <  2:46244/73‑1 (MQ=255)                                                       cgcgAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                >  2:829993/1‑76 (MQ=255)                                                          gAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggcagagg             >  2:451128/1‑76 (MQ=255)                                                                ccGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAag       <  2:885991/76‑1 (MQ=255)                                                                  gCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAagag     >  1:926503/1‑76 (MQ=255)                                                                   cTGGCTATTCCCAGTACGCCGATCCCGCTGGGATAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAgg    >  2:1035263/1‑76 (MQ=255)                                                                    tGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGt   <  2:575473/76‑1 (MQ=255)                                                                     ggCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTg  >  2:960439/1‑76 (MQ=255)                                                                        |                                                                         GAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTG  >  REL606/4183988‑4184131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑