Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,194,420	G→T	V232V (GTG→GTT)	metA →	homoserine O‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,194,420	0	G	T	100.0%	199.6 / NA	52	V232V (GTG→GTT)	metA	homoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/26);  total (26/26)

TGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAC  >  REL606/4194347‑4194492                                                                          |                                                                         tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTAc                                                                        <  1:894732/76‑1 (MQ=255)  ggAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACg                                                                       <  1:370871/76‑1 (MQ=255)      aTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGcc                                                                   >  2:1182272/1‑76 (MQ=255)        tCTGGCAGAGTCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCAt                                                                 >  1:1120035/1‑76 (MQ=255)        gcaggCAGAGACGNAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCAt                                                                 <  1:160576/73‑1 (MQ=255)         cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATc                                                                <  2:1067543/76‑1 (MQ=255)          tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATcc                                                               >  1:791206/1‑76 (MQ=255)          tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATcc                                                               <  1:547368/76‑1 (MQ=255)             cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGa                                                            <  1:1115724/76‑1 (MQ=255)             cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATACCGa                                                            <  1:73418/76‑1 (MQ=255)              agagACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGaa                                                           >  1:676500/1‑76 (MQ=255)               gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAat                                                          >  2:86749/1‑76 (MQ=255)                agaCGGAAGAAGGTGATGCATATCTGTTTGCCANTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAata                                                         >  2:1020586/1‑76 (MQ=255)                    ggAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATatgat                                                     <  1:912268/76‑1 (MQ=255)                     gaggaagGGGATGCATATCTGTTTGCCAGT‑AAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgc                                                   <  2:618917/73‑1 (MQ=255)                     gaagaaGGGGATGCATATCTCTNNGCTAGTAAAGNNTAGCGCATTGCCTTGGTTACGGGCCATCCCGAATatgatg                                                    <  1:1093043/76‑1 (MQ=255)                      aagaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgc                                                   <  1:763073/76‑1 (MQ=255)                       agaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgcg                                                  >  1:382223/1‑76 (MQ=255)                        gaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgcgc                                                 <  1:495703/76‑1 (MQ=255)                         aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa                                                <  1:944064/76‑1 (MQ=255)                         aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa                                                <  1:1174909/76‑1 (MQ=255)                         aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa                                                >  1:652491/1‑76 (MQ=255)                          agGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCaa                                               <  2:989999/76‑1 (MQ=255)                           ggggATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCaaa                                              <  2:65858/76‑1 (MQ=255)                                 gCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTgg                                        >  1:783547/1‑76 (MQ=255)                                   atatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg                                      >  1:781954/1‑76 (MQ=255)                                   atatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg                                      <  1:461167/76‑1 (MQ=255)                                       cTGTGTTCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCTAATATGATGCGCAAACGCTGGCGCAgg                                  <  1:1199284/76‑1 (MQ=255)                                          tttGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAt                               >  1:1170948/1‑76 (MQ=255)                                                aGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTcc                         <  2:219897/76‑1 (MQ=255)                                                 gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttttacc                        >  1:943793/1‑73 (MQ=255)                                                 gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg                        >  1:943825/1‑76 (MQ=255)                                                 gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg                        >  2:574878/1‑76 (MQ=255)                                                  tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc                       >  1:836941/1‑76 (MQ=255)                                                  tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc                       >  2:741384/1‑76 (MQ=255)                                                  tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc                       >  2:671833/1‑76 (MQ=255)                                                  tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc                       >  2:540644/1‑76 (MQ=255)                                                   aaaGATAAGNGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg                      >  1:30353/1‑76 (MQ=255)                                                   aaaGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg                      >  1:643776/1‑76 (MQ=255)                                                   aaaGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg                      >  2:722225/1‑76 (MQ=255)                                                      gATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtg                   <  1:722848/76‑1 (MQ=255)                                                      gATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtg                   >  2:916180/1‑76 (MQ=255)                                                        tAAGCGTATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg                 >  1:71066/1‑76 (MQ=255)                                                        tAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg                 <  2:237741/76‑1 (MQ=255)                                                        tAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgta                 <  2:1077426/76‑2 (MQ=255)                                                           gcgcATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGaa              <  1:695987/76‑1 (MQ=255)                                                             gcATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGc            <  1:859433/76‑1 (MQ=255)                                                               aTTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg          <  2:976622/76‑1 (MQ=255)                                                                  gCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCCCGATGTGGAAGCCGGAc       >  1:126361/1‑76 (MQ=255)                                                                   ccTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt      <  1:833921/76‑1 (MQ=255)                                                                     tttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg    >  2:266036/1‑76 (MQ=255)                                                                      ttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa   <  1:444378/76‑1 (MQ=255)                                                                      ttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa   >  1:487892/1‑76 (MQ=255)                                                                       tgttACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAc  >  2:1079715/1‑76 (MQ=255)                                                                          |                                                                         TGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAC  >  REL606/4194347‑4194492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑