Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,536	G→T	A235E (GCG→GAG)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,536	0	G	T	100.0%	103.0 / NA	28	A235E (GCG→GAG)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/17);  total (11/17)

AATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCA  >  REL606/1305466‑1305608                                                                       |                                                                         aaTCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATg                                                                     >  2:632203/1‑76 (MQ=255)       ttGCNCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCNACCATATTCATGCTGCCGGTGTACTCGATGTAATTa                                                               <  1:138814/76‑1 (MQ=255)           ccTACGCCCGCATCTTGTTTGAAGTAGCGAGGGNCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCga                                                           >  2:1028523/1‑76 (MQ=255)               cGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCgatcat                                                       <  1:559479/76‑1 (MQ=255)               cGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCgatcat                                                       <  2:361164/76‑1 (MQ=255)                  ccGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCgatcatgat                                                    >  1:792487/1‑76 (MQ=255)                     cATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCgatcatgatcat                                                 >  2:456837/1‑76 (MQ=255)                            tttGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGa                                          >  1:807769/1‑76 (MQ=255)                              tGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATg                                        >  1:331631/1‑76 (MQ=255)                               gAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGCGTACTCGATGTAATTACCGATCATGATCATCTTGCGATGt                                       >  2:417437/1‑76 (MQ=255)                                aaGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGtt                                      >  1:316165/1‑76 (MQ=255)                                aaGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGtt                                      <  1:510762/76‑1 (MQ=255)                                aaGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGtt                                      >  2:648778/1‑76 (MQ=255)                                  gTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTgg                                    <  1:630341/76‑1 (MQ=255)                                     gCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgcgc                                 <  2:64700/76‑1 (MQ=255)                                     gCGAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGgcgc                                 <  2:338155/76‑1 (MQ=255)                                       gAGGGTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAg                               <  2:261250/76‑1 (MQ=255)                                          ggTCGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTc                            <  1:1071254/76‑1 (MQ=255)                                             cGACCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCAt                         <  2:835268/76‑1 (MQ=255)                                               aCCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                       <  2:410761/76‑1 (MQ=255)                                               aCCATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATAc                       <  1:667884/76‑1 (MQ=255)                                                ccATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACg                      <  2:56070/76‑1 (MQ=255)                                                 cATATTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACgg                     <  2:517120/76‑1 (MQ=255)                                                    atTCATGCTGCCGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGt                  <  2:891361/76‑1 (MQ=255)                                                               cGGTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACg       <  2:322990/76‑1 (MQ=255)                                                                ggTGTACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGc      >  1:933148/1‑76 (MQ=255)                                                                   gtACTCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCAta   >  2:840724/1‑75 (MQ=255)                                                                    tACTCGATNTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCa  <  1:1065911/76‑1 (MQ=255)                                                                       |                                                                         AATCCATTGCCCTACGCCCGCATCTTGTTTGAAGTAGCGAGGGTCGACCATATTCATGCTGCCGGTGTACGCGATGTAATTATCGATCATGATCATCTTGCGATGTTGGCGCAGGTCCATACGGCGTAAAAACACACGCATCA  >  REL606/1305466‑1305608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑