Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,516	0	C	T	100.0%	100.3 / NA	28	H33Y (CAC→TAC)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

AGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329445‑1329588                                                                        |                                                                         aGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACAt                                                                      <  2:755181/76‑1 (MQ=255)     cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCcgc                                                                  <  1:250824/76‑1 (MQ=255)      ccGGCAAAAGCCAAAACGATCAACAAGTATATGGGTAGTGACTACGTGGTGAAATCCAGCGTNGGTTACATCcgcg                                                                 >  2:1101879/1‑76 (MQ=255)            aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:325738/1‑76 (MQ=255)            aaaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGc                                                           >  2:325713/1‑76 (MQ=255)                        aTCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTCCCGACCAGTGGCt                                               <  1:78911/76‑1 (MQ=255)                         tCAACAAGTATCTGGGTAGTGACTACGTGGTGAAAGCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTc                                              <  1:263376/76‑1 (MQ=255)                            acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGc                                           >  2:261655/1‑76 (MQ=255)                             caaGTATCTGGGTAGTGACTACGTGGTGAAATCCTGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCt                                          <  1:774127/76‑1 (MQ=255)                              aaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTg                                         <  1:350940/76‑1 (MQ=255)                                 tATCTGGGTAGTGACTACGTTGAGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCa                                      >  1:8703/1‑76 (MQ=255)                                  aTCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCNGCTGCCaa                                     >  1:1069653/1‑76 (MQ=255)                                   gcggggTAGTGACNACGTGGTGAACTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaa                                    <  1:162617/73‑1 (MQ=255)                                    ntGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGNCTCAGCTGCCaaaa                                   >  2:167684/2‑76 (MQ=255)                                     tGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaaa                                  >  1:393984/1‑76 (MQ=255)                                         tAGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGt                              <  2:107398/76‑1 (MQ=255)                                          aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             <  2:523059/76‑1 (MQ=255)                                          aGTGACTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                             >  2:347906/1‑76 (MQ=255)                                               cTACGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGAc                        >  1:879838/1‑76 (MQ=255)                                                  cGTGGTGAAATCCAGCGTCGGTTACATCCGCGATTTGCCGACCATTGGATCAGCTGCCCAAAAGAGTGCCGActct                     >  2:188030/1‑76 (MQ=255)                                                       tgAAATCCAGCGTCGGTTACATCCGTGATTTACCGACCATNNGCTCAGCTGCNNACAAGAGTGCCGACGCTACCTc                >  1:1083683/1‑76 (MQ=255)                                                         aaaTCCAGCGTCGGTTACATCCGCGATTTGCCGATCCGGGGCTCAGCTGCAGAAAAGAGAGCCGACTCTACCTccc              >  2:675254/1‑75 (MQ=255)                                                          aaTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTccac             <  1:295929/76‑1 (MQ=255)                                                           aTCCAGCGTCGGTTACATCCGCGATTTGCCGACCAGNGGCTCACGTGCCAAACAGAGTGCCGACTCTACCTncacc            >  1:140744/1‑76 (MQ=255)                                                             ccAGCGTCGGTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCaa          >  1:951111/1‑76 (MQ=255)                                                                  gTCGGTGACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAATAGAGTGCCGACTCTACCTCCACCAAGACgg     <  1:1101880/76‑1 (MQ=255)                                                                     ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:348551/76‑1 (MQ=255)                                                                     ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:136121/76‑1 (MQ=255)                                                                     ggTTACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTa  <  2:885685/76‑1 (MQ=255)                                                                        |                                                                         AGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTA  >  REL606/1329445‑1329588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑