Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,125,562	IS186 (+) +6 bp	coding (131‑136/2544 nt)	mdoH →	glucosyltransferase MdoH

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	1125562 =	1 (0.030)	40 (1.000)	29/146	0.3	97.4%	coding (131/2544 nt)	mdoH	glucosyltransferase MdoH
	?	REL606	= 1858406	NA (NA)					noncoding (1343/1343 nt)	IS186	repeat region
*	?	REL606	= 1125567	1 (0.030)	45 (1.140)	34/144	0.1	97.6%	coding (136/2544 nt)	mdoH	glucosyltransferase MdoH
	?	REL606	1857064 =	NA (NA)					noncoding (1/1343 nt)	IS186	repeat region

GCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1125494‑1125567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT  >  REL606/1857064‑1857138                                                                                                                                                       GCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCC                                                                           >  1:231883/1‑76   CGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCA                                                                         <  2:19676/76‑1     AAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATA                                                                       <  2:339237/76‑1     AAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATA                                                                       >  2:118876/1‑76      AGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAA                                                                      >  1:1080986/1‑76      AGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAA                                                                      >  2:1038893/1‑76       GACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAG                                                                     >  2:1025911/1‑76        ACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGC                                                                    >  1:304862/1‑76         CTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCG                                                                   >  2:986562/1‑76            ATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTA                                                                >  2:625061/1‑76            ATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTA                                                                >  1:882522/1‑76             TATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAA                                                               >  1:386358/1‑76             TATCCGCGCCGTTAATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAA                                                               <  1:1128065/76‑1              ATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAAC                                                              >  2:672620/1‑76                   CGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAG                                                         >  1:460887/1‑76                     CCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGNGCTAACTTAAcgc                                                       >  1:175248/1‑73                        TTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTG                                                    <  2:487263/76‑1                        TTCATCAGGCGCTGGAAGCCGAACACCTCACCTGGGCGCGGGAGGACGATTCCCATAAGCGCTAACTTAAGGGTTG                                                    >  1:66259/1‑76                         TCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGT                                                   >  2:1154060/1‑76                               GGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATT                                             <  2:555729/76‑1                                GCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTA                                            <  1:893019/76‑1                                 CGCNGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCNCATAAGCGCTAACTTAAGGGTTGTGGTATTAC                                           <  2:1234360/76‑1                                  GCTGGATGCCGAACACCGCACCTGGGCGCGGNAGGATGATGCCCATNAGCGCNAACTTAAGGGTTGCGGTATTACG                                          <  2:164595/76‑1                                   CTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                         >  2:671626/1‑76                                     GGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                       >  1:564089/1‑76                                     GGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                       >  2:597667/1‑76                                     GGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                       >  2:224885/1‑76                                           CGAACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATG                                 >  2:723001/1‑76                                             AACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAT                               >  2:1038584/1‑76                                             AACACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAT                               >  1:365001/1‑76                                               CACCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                             >  2:847192/1‑76                                                 CCGCACCTGGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAA                           <  1:625061/76‑1                                                   actCCTGGGCGCGNGAGGATGATTACCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACG                         <  1:176851/73‑1                                                         GGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGA                   >  2:1001415/1‑76                                                         GGGCGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGA                   <  1:1117865/76‑1                                                            CGCGGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGA                >  2:679153/1‑76                                                               GGGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATT             <  1:220143/76‑1                                                                GGAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTA            >  1:681723/1‑76                                                                 GAGGATGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAC           >  1:711968/1‑76                                                                      TGATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCA      >  2:576430/1‑76                                                                       GATTCCCATAAGCGCTAACTTAA‑GGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAAATACTCTCACG    <  1:1214566/76‑1                                                                        ATTCCCCTAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG    <  1:1294439/76‑1                                                                        ATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG    <  1:1001949/76‑1                                                                        ATTCCCATAAGCGCTAACTTAAGGATTGGGGNATTACGCCGGATATNATTTANCGTGCCGATGAATGACTCTCACG    <  2:175248/76‑1                                                                         TTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGA   <  1:269607/76‑1                                                                          TCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT  <  1:658506/76‑1                                                                                                                                                       GCCGAAGACTGATATCCGCGCCGTTCATCAGGCGCTGGATGCCGAACACCGCACCTGGGCGCGGGAGGATGATTccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1125494‑1125567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT  >  REL606/1857064‑1857138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.