| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,492,082 | 0 | T | G | 35.9% | 88.1 / 17.3 | 53 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (6/28); new base G (18/1); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.31e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAC > REL606/3492009‑3492156 | gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtgg < 2:536146/76‑1 (MQ=255)gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggg > 1:1285178/1‑76 (MQ=255) tgtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTTTGAGTCAGTGGCTGGTGGAGta < 1:1047853/76‑1 (MQ=255) tgtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGta > 1:1176666/1‑76 (MQ=255) ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATAc < 1:375973/76‑1 (MQ=255) gggTCGGACAAAACGAGGCATGGGGCGAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACg < 1:524204/74‑1 (MQ=255) gcgcCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACg < 1:524179/76‑1 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgc < 2:463139/76‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgca > 2:912638/1‑76 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGGATACgca > 2:236641/1‑76 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagc < 1:1026508/76‑1 (MQ=255) cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgcagc > 1:1144604/1‑76 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACgcaggg > 2:350327/1‑74 (MQ=255) acaAAACAAGGCATGTGGCTAGATTCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGa < 1:1238258/76‑1 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAgg > 2:994447/1‑75 (MQ=255) aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGAAGCt > 2:815873/1‑76 (MQ=255) aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCt < 1:594206/76‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTg < 2:412190/76‑1 (MQ=255) cAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGt > 1:366072/1‑76 (MQ=255) cAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGt > 2:862887/1‑76 (MQ=255) aaGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTc < 1:564023/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTgg < 1:298738/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTgg < 2:473772/76‑1 (MQ=255) aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTgg > 2:1299861/1‑76 (MQ=255) tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGc < 1:922025/76‑1 (MQ=255) tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGGCGGTGGc > 2:1043759/1‑76 (MQ=255) gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGcg < 2:25718/76‑1 (MQ=255) gCTAAAGGCGGATCAACAATGTNNTGTGAGTCAGNNCCTGGTGGAGTATATGCAGCAGAAGCTGTCGCTGGcgtcg < 1:1185453/76‑1 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATAAGCAGGAGAAGCTTTCGCTGgcggcgg > 1:256233/1‑75 (MQ=255) cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCGGgcggcgg > 1:750015/1‑75 (MQ=255) aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTgg > 2:429637/1‑75 (MQ=255) aTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGAtt < 1:512490/76‑1 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGGtttt > 2:999118/1‑75 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGATTTaa > 2:26089/1‑76 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGCGGCGGGGtttta > 2:218980/1‑74 (MQ=255) cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGAtttta > 2:1175874/1‑74 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa < 1:312796/76‑1 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa < 1:312817/76‑1 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa < 1:393368/76‑1 (MQ=255) tCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGGGATTTTATGTa > 1:817636/1‑76 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGTGGGGGATACGCCGCAGAAGCTGTCGCTGGCGGCGTGGTTTTATGTAAg > 2:693864/1‑76 (MQ=255) aacaaTCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGGCGTGGTTTAATGTAAg > 2:725962/1‑76 (MQ=255) acaaTCTCGTGTGAGTCAGTGGCTGGTGNAGTATACGCGTCCGAAGCAGTCGCTGGCGTCGTGATTTAATGTAAgg < 1:113601/76‑1 (MQ=255) acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAgg < 1:791670/76‑1 (MQ=255) aaaaTCTCGTGTGGGTCGGGGGCTGGGGGAGTATACGCAGCGGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAgg < 1:791669/74‑1 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGt < 2:765120/76‑1 (MQ=255) aaTCTCGTGTGACTCAGTGGCTGGGGGAGGATACGCAGCAGAAGCTGGCGCTGGCGGCGGGGTTTTATTTAAGGTg > 2:459978/1‑76 (MQ=255) ctcGTGTGAGTCAGTGGGTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGcc < 1:141027/76‑1 (MQ=255) ctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGGGGcc > 1:1017586/1‑76 (MQ=255) tcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCa < 2:778882/76‑1 (MQ=255) gtgtGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGCGGCGTGGTTTTATGTAAAGGGGGCAgg > 2:1054940/1‑76 (MQ=255) tgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCa < 1:557371/76‑1 (MQ=255) gAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAg < 2:1152523/76‑1 (MQ=255) tCAGTGGCTGGGGGAGGATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGGGGGCAGGGCCGAtt > 2:472308/1‑76 (MQ=255) aGTGGCTGGGGGGGGATACGCAGCAGAAACTGTCGCTGGGGGGGTAGTTTTATTTAAGGGGGNCAGGGGAGATTTc > 2:1197676/1‑76 (MQ=255) gTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCa < 1:182117/76‑1 (MQ=255) gTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCa < 1:906357/76‑1 (MQ=255) cgacTGGTGGAGTNTACGCAGCAGGAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCaa < 1:174142/73‑1 (MQ=255) ggCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAAt < 2:739732/76‑1 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt > 2:988044/1‑76 (MQ=255) cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGGCAGATTTTAATCt > 1:289912/1‑76 (MQ=255) tggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATNTAATGTAAGGTGGCCAGGTCAGATTTCAATCTg < 2:1113548/76‑1 (MQ=255) tggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATCTAATGTAAGGTGGCCAGGTCAGAGTTCAATCTg < 2:1129079/76‑1 (MQ=255) gtggAGTATACGCAGCAGAAGCTGTCGNTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAc > 1:1309895/1‑76 (MQ=255) | GCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAC > REL606/3492009‑3492156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |