| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,094 | G→T | G747C (GGT→TGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,094 | 0 | G | T | 95.6% | 154.5 / ‑3.6 | 45 | G747C (GGT→TGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (26/17); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTG > REL606/4163024‑4163166 | cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATc > 1:606289/1‑76 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCg < 2:820960/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCg < 1:356227/76‑1 (MQ=255) cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACa > 1:400042/1‑76 (MQ=255) gTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATc < 1:568814/76‑1 (MQ=255) aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTa > 1:201504/1‑76 (MQ=255) tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACaa > 2:1287911/1‑76 (MQ=255) ggATGCTTGCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAAc > 1:604952/1‑76 (MQ=255) ggATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAAc < 2:103438/76‑1 (MQ=255) gATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAAcc < 2:313663/76‑1 (MQ=255) aTGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCt < 2:362108/76‑1 (MQ=255) tGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTg < 1:306155/76‑1 (MQ=255) gCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGa > 1:1212487/1‑76 (MQ=255) ttCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTANAACCTGAcc < 1:34921/76‑1 (MQ=255) ttCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTANAACCTGAcc < 1:33180/76‑1 (MQ=255) ttCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGAcc > 2:1174643/1‑76 (MQ=255) cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCaaaa > 1:584638/1‑75 (MQ=255) cGTTATCATAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACAcc > 1:1047999/1‑76 (MQ=255) cGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACAcc > 1:752611/1‑76 (MQ=255) ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCg > 2:119552/1‑76 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGtt > 2:615164/1‑76 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc < 2:272146/76‑1 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc > 1:259432/1‑76 (MQ=255) tAACGAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAg > 1:1281360/1‑76 (MQ=255) cGAAGACGAAATGTATCCGGGTGAAGCATGTATGGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAac > 2:1131781/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca > 2:989749/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca > 2:537540/1‑76 (MQ=255) aGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACAcc > 2:81618/1‑76 (MQ=255) aGACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACAcc < 2:263933/76‑1 (MQ=255) gACGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCt < 2:1212486/76‑1 (MQ=255) aCGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg > 1:687000/1‑76 (MQ=255) cGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGt < 1:133843/76‑1 (MQ=255) cGAGATGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGt < 1:310868/76‑1 (MQ=255) gagaTGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTa > 1:192364/1‑76 (MQ=255) gaTGTATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATc > 1:656843/1‑76 (MQ=255) gaTGCAGCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATATACCCGTTCTAACCAGAACACCTGTATc < 1:1294227/76‑1 (MQ=255) aTGTATCCGGGTGAAGCATGTATCGACCTCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCAGAATCa > 1:1304218/1‑76 (MQ=255) tATCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc > 1:423208/1‑76 (MQ=255) tCCGGGTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAg < 2:162921/76‑1 (MQ=255) ggTGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc > 1:109763/1‑76 (MQ=255) tGAAGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCg < 1:912924/76‑1 (MQ=255) aaGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:765802/1‑76 (MQ=255) aaGCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:765812/1‑76 (MQ=255) aaGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg < 2:235119/76‑1 (MQ=255) gCATGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtgtg > 1:958645/1‑76 (MQ=255) gCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCgggtg < 2:709531/76‑1 (MQ=255) | CGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTG > REL606/4163024‑4163166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |