Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	84.1 / NA	27	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/15);  total (12/15)

CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851                                                                        |                                                                         cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  1:1283552/1‑76 (MQ=255)      cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  1:968633/76‑1 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                >  1:354849/1‑76 (MQ=255)       aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                                <  1:844704/76‑1 (MQ=255)        tCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGAt                                                               >  1:313299/1‑76 (MQ=255)                       cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                                <  1:434281/76‑1 (MQ=255)                          cTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATCAAGCATGACGCCTGTAt                                             >  1:567889/1‑76 (MQ=255)                            ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                           >  2:701820/1‑76 (MQ=255)                               cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  2:771001/76‑1 (MQ=255)                               cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        <  1:305164/76‑1 (MQ=255)                                 ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  1:521195/76‑1 (MQ=255)                                        ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  1:242786/76‑1 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:466006/76‑1 (MQ=255)                                         gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:1170717/76‑1 (MQ=255)                                             tCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                          >  1:515751/1‑76 (MQ=255)                                               aGCCNCAATAACAGCAAAACGCCGATCCGCATCAGATAANGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  1:152232/76‑1 (MQ=255)                                               aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                        <  2:567889/76‑1 (MQ=255)                                                 ccccAATAACAGCANAACGCCAATCCCCACCAGANAAAGCGTGNCGCCTGTATTAAACAGGCTCTTTACCGATGgc                      <  2:1084627/76‑1 (MQ=255)                                                  cccAATAACAGCAAAACGCCAATCCCCACCAGATACAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcg                     >  2:267474/1‑76 (MQ=255)                                                        aaCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgc               >  1:200892/1‑76 (MQ=255)                                                          cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             >  2:249810/1‑76 (MQ=255)                                                          cAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc             <  2:80364/76‑1 (MQ=255)                                                            gCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAc           <  1:982751/76‑1 (MQ=255)                                                             cAAACCGCCAACCCCCCCCAGATAAAGCATGACGCCTGTATTTAACAGGCTTTTTCCCGATGGCGCATCGCGCAcc          <  2:1135017/76‑1 (MQ=255)                                                                   gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  2:711625/1‑76 (MQ=255)                                                                   gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc    >  2:895678/1‑76 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGCTGGCGCATCGNGCACCAgccgcct  >  1:166759/1‑75 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgccc  >  1:723038/1‑75 (MQ=255)                                                                     cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  <  2:18926/76‑1 (MQ=255)                                                                        |                                                                         CGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193708‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑