| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,504,889 | (A)8→7 | intergenic (+633/‑92) | insB‑25 → / → ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,504,882 | 0 | A | . | 92.7% | 287.1 / 18.9 | 68 | intergenic (+626/‑99) | insB‑25/ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base . (31/32); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCAAAA > REL606/4504807‑4504951 | aGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTcaca < 1:219852/76‑1 (MQ=255)aGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTcaca > 2:1616687/1‑76 (MQ=255) gATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaa > 2:49908/1‑76 (MQ=255) gATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaa > 2:791468/1‑76 (MQ=255) aTAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaa > 1:502737/1‑76 (MQ=255) aTAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaa < 2:1124594/76‑1 (MQ=255) aaTGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaaa > 1:1443857/1‑76 (MQ=255) ttttAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACaaaaaaacca > 1:335700/1‑73 (MQ=255) ttAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCAtt < 2:390594/76‑1 (MQ=255) aGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAg < 2:1170057/76‑1 (MQ=255) gTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGt < 1:1284287/76‑1 (MQ=255) tAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTc < 1:1432619/76‑1 (MQ=255) tAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTc < 1:1139869/76‑1 (MQ=255) aaaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCt < 1:1247942/76‑1 (MQ=255) aaaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCt > 1:1076374/1‑76 (MQ=255) aaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTg < 1:36632/76‑1 (MQ=255) aaaaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTg < 1:36627/76‑1 (MQ=255) aaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCt < 1:393905/76‑1 (MQ=255) aaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCt < 1:612494/76‑1 (MQ=255) aaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCt < 1:612504/76‑1 (MQ=255) aaaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCt > 2:938303/1‑76 (MQ=255) aaGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCtt < 1:1393420/76‑1 (MQ=255) agTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCt > 1:44217/1‑76 (MQ=255) agTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCt < 2:807397/76‑1 (MQ=255) agTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCt < 2:807412/76‑1 (MQ=255) gTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTg < 2:975772/76‑1 (MQ=255) gTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTg < 2:113731/76‑1 (MQ=255) gTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTg < 2:113738/76‑1 (MQ=255) tGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGtt < 2:258711/76‑1 (MQ=255) tGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGtt < 2:1210787/76‑1 (MQ=255) tGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTa < 2:295067/76‑1 (MQ=255) tGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTa < 1:1609211/76‑1 (MQ=255) gAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTa > 1:757664/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAA‑CATTAGTCTGCTTCCTGTTCTTACa < 1:204522/76‑1 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAA‑CATTAGTCTGCTTCCTGTTCTTACa < 1:204513/76‑1 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 2:196599/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 1:1182591/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 1:898588/1‑76 (MQ=255) aaTATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACa > 1:898609/1‑76 (MQ=255) tataATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaat < 2:1396551/76‑1 (MQ=255) taataCAGCCTCTTCCTACATANGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa > 2:231879/1‑76 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACaata > 1:610557/1‑76 (MQ=255) taataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataa < 1:1502036/76‑1 (MQ=255) aataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataat > 2:670437/1‑76 (MQ=255) aataCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACaataat < 1:1283734/76‑1 (MQ=255) taCAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATc < 2:677210/76‑1 (MQ=255) taCAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCa > 2:72027/1‑76 (MQ=255) cAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGt < 1:951052/76‑1 (MQ=255) cAGCCTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGt < 1:327557/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCGC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 2:292070/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 1:1154616/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 1:327816/76‑1 (MQ=255) ccTCTTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatt < 1:273436/76‑1 (MQ=255) ctctTCCTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGtatta > 1:824254/1‑76 (MQ=255) cTACATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatt > 1:1170391/1‑76 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt > 2:1179869/1‑76 (MQ=255) aCATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttt > 2:70426/1‑76 (MQ=255) cATAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtatttta > 1:134501/1‑76 (MQ=255) aTAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttat > 2:303163/1‑76 (MQ=255) aTAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttat > 2:304686/1‑76 (MQ=255) tAAGAAGGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttatt > 2:1148839/1‑76 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt > 2:512742/1‑76 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt > 2:972426/1‑76 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt < 2:628550/76‑1 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt > 2:333879/1‑76 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt > 2:333866/1‑76 (MQ=255) aagaagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGtattttattt < 2:26620/76‑1 (MQ=255) aagGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtata > 2:1177258/1‑76 (MQ=255) agGAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatat > 2:33126/1‑76 (MQ=255) ggAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTtatata > 1:144208/1‑76 (MQ=255) gAATGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAg > 1:1581207/1‑76 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc > 2:1127713/1‑76 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc > 2:1127702/1‑76 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc > 2:932907/1‑76 (MQ=255) aaTGTGCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGc > 2:1385801/1‑76 (MQ=255) tgCTCAC‑AAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCaaaa > 1:806076/1‑76 (MQ=255) | AGATAATGTTTTTAGTAAAAAAGAGTTGACTGAATATAATACAGCCTCTTCCTACATAAGAAGGAATGTGCTCACAAAAAAAACCATTAGTCTGCTTCCTGTTCTTACAATAATCAGTATTATGTATTTTATTTATATAGCAAAA > REL606/4504807‑4504951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |