Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606154,4970TG28.8% 114.5 / 10.9 52A191A (GCA→GCC) yadLpredicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (4/33);  new base G (15/0);  total (19/33)
Fisher's exact test for biased strand distribution p-value = 8.65e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA  >  REL606/154423‑154572
                                                                          |                                                                           
aCGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATANGTTACTGTAAATGTGGCAGGTg                                                                            <  2:232918/76‑1 (MQ=255)
 cGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGc                                                                           <  1:762392/76‑1 (MQ=255)
  gTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCa                                                                          <  1:1249727/76‑1 (MQ=255)
  gTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGcg                                                                          >  1:249264/1‑75 (MQ=255)
   ttGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAg                                                                         <  1:51872/76‑1 (MQ=255)
   ttGAGTGGGATGCATATATAGTTCCTGGACCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAg                                                                         >  1:968138/1‑76 (MQ=255)
     gAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGaa                                                                       >  1:54823/1‑76 (MQ=255)
      aGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaa                                                                      >  1:221237/1‑76 (MQ=255)
       gTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGaaaa                                                                     >  1:79376/1‑76 (MQ=255)
          ggATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAatt                                                                  >  1:1466424/1‑76 (MQ=255)
           gATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTa                                                                 <  1:143961/76‑1 (MQ=255)
                     taGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGa                                                       <  2:64462/76‑1 (MQ=255)
                     taGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTACCCGCAGGGa                                                       >  1:383240/1‑76 (MQ=255)
                      aGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGAc                                                      <  1:1325011/76‑1 (MQ=255)
                        ttCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAAATTACCCGCCGTGAcct                                                    >  1:350834/1‑74 (MQ=255)
                        ttCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCCGTGGCAt                                                    >  1:1407710/1‑76 (MQ=255)
                         tCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACAtt                                                   <  1:1093185/76‑1 (MQ=255)
                          ccTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTg                                                  <  2:507006/76‑1 (MQ=255)
                          ccTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTg                                                  <  1:16053/76‑1 (MQ=255)
                           cTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCCGTGACATTGc                                                 >  2:1032910/1‑76 (MQ=255)
                            tGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCCCAGTGACATTTCt                                                >  1:510676/1‑76 (MQ=255)
                             ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCtt                                               <  2:1666831/76‑1 (MQ=255)
                             ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCtt                                               <  1:1068744/76‑1 (MQ=255)
                             ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCtt                                               <  1:15693/76‑1 (MQ=255)
                             ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCCGTGACATTGttt                                               >  1:480818/1‑76 (MQ=255)
                             ggATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGGGACATTGCtt                                               >  2:326913/1‑76 (MQ=255)
                                tCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCg                                            >  2:1408056/1‑76 (MQ=255)
                                tCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTACCCGCAGTGACATTGCTTACg                                            >  1:1026348/1‑76 (MQ=255)
                                  tGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGt                                          >  1:1342803/1‑76 (MQ=255)
                                    tCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGttt                                        >  2:412129/1‑76 (MQ=255)
                                     cAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtttt                                       <  2:1621626/76‑1 (MQ=255)
                                      aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTa                                      <  2:90625/76‑1 (MQ=255)
                                       gTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAt                                     <  2:1667802/76‑1 (MQ=255)
                                          tGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAtttt                                  <  1:1226131/76‑1 (MQ=255)
                                          tGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTAACCCCCGGGAAATTGCTTTCGGTtttttttt                                  >  1:181416/1‑76 (MQ=255)
                                            ttagttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAtttttt                                <  2:1562724/76‑1 (MQ=255)
                                                 tataGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTcgac                           <  1:1507127/76‑1 (MQ=255)
                                                  ataGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTcgacg                          <  2:21759/76‑1 (MQ=255)
                                                  ataGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTAACCGCAGTGACATTGCTTTCGGTTTTTTTTTTTTtgacg                          >  1:1350414/1‑76 (MQ=255)
                                                    aGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCgacgac                        <  1:507155/76‑1 (MQ=255)
                                                     ggTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGAcc                       <  2:245425/76‑1 (MQ=255)
                                                      gTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCa                      <  2:1674048/76‑1 (MQ=255)
                                                          cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg                  <  2:115027/76‑1 (MQ=255)
                                                          cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg                  <  2:249264/76‑1 (MQ=255)
                                                          cTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTTTTTTTTTCGACGACCCGTCg                  >  1:123169/1‑76 (MQ=255)
                                                          cTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGGGACATTGCTTACGGGTTTATTTTTTTCGACGACCAGTCg                  >  1:1325704/1‑76 (MQ=255)
                                                             tAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGc               <  1:292315/76‑1 (MQ=255)
                                                             tAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGc               <  1:1172371/76‑1 (MQ=255)
                                                             tAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGc               <  1:1172378/76‑1 (MQ=255)
                                                             tAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGc               <  2:1161469/76‑1 (MQ=255)
                                                              aaaTGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCa              <  2:158893/76‑1 (MQ=255)
                                                                   tgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCAt         >  1:1497315/1‑76 (MQ=255)
                                                                   tgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCAt         <  1:975366/76‑1 (MQ=255)
                                                                       aGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGa     <  1:555800/76‑1 (MQ=255)
                                                                       aGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGa     >  1:929072/1‑76 (MQ=255)
                                                                         gTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGata   <  1:933623/76‑1 (MQ=255)
                                                                          tGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGataa  >  2:835872/1‑76 (MQ=255)
                                                                          |                                                                           
ACGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA  >  REL606/154423‑154572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑