| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,484,105 | 0 | A | G | 37.1% | 42.6 / 19.3 | 35 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/18); new base G (13/0); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484034‑1484180 | ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttt > 1:1437043/1‑76 (MQ=255) gctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtat < 1:1678220/76‑1 (MQ=255) ctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt < 1:803794/76‑1 (MQ=255) tGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtttt < 1:1307837/76‑1 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 1:1315336/76‑1 (MQ=255) ccACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg < 1:829822/76‑1 (MQ=255) cACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTATTTTTGTCGa > 1:1600666/1‑76 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGAttt > 1:497664/1‑76 (MQ=255) tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTAATTTTTACGAttt > 2:550695/1‑76 (MQ=255) aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGACAGGGGTTTTTTAATTTTTGAGATTTc > 2:747870/1‑76 (MQ=255) aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCa > 1:71256/1‑76 (MQ=255) gtgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc < 1:95088/76‑1 (MQ=255) ggTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTACCAt > 1:144065/1‑76 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTAGGATTTTACCCTGt > 2:1176840/1‑76 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATGt > 1:1248455/1‑76 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGt > 1:1518819/1‑76 (MQ=255) cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTAATTTTTTCGATTTTCCCATGTCggga > 1:346049/1‑74 (MQ=255) cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTa < 2:1311297/76‑1 (MQ=255) cATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTa < 2:465764/76‑1 (MQ=255) aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa < 1:374989/76‑1 (MQ=255) ttGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATTTCGGTaaa > 1:1199478/1‑75 (MQ=255) tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGcc < 1:470500/76‑1 (MQ=255) cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCg < 2:1061691/76‑1 (MQ=255) ttCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAAc < 1:373393/76‑1 (MQ=255) ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGa < 1:896161/76‑1 (MQ=255) ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGa < 1:227722/76‑1 (MQ=255) gCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTTTTTTGACGGTTTCACCATGTCGGGAACGCCGGAACTGAc > 1:243604/1‑76 (MQ=255) cGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCAAGTCCGGAACGCCGGGACTGACGcg > 2:65329/1‑75 (MQ=255) cGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTTACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt > 2:1489543/1‑76 (MQ=255) cGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt > 2:117742/1‑76 (MQ=255) tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc < 1:1150583/76‑1 (MQ=255) gctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAAGCCGGGACTGACGCTGGCg > 2:1666158/1‑76 (MQ=255) tgACACTGTTATGGCAGGGATTGTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGaa < 1:699100/76‑1 (MQ=255) gTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGTCGGTAACGCCGGAAATGACGCTCGCGAATATCCgg > 1:1160236/1‑75 (MQ=255) tGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcgc > 2:380898/1‑76 (MQ=255) ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt < 1:1303466/76‑1 (MQ=255) gggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGttt < 2:193002/76‑1 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt < 1:831922/76‑1 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt < 1:613644/76‑1 (MQ=255) | CTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484034‑1484180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |