| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,983,700 | 0 | T | G | 44.4% | 32.2 / 20.9 | 36 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (6/14); new base G (16/0); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTAAATGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCAGTAATTGC > REL606/3983629‑3983775 | tgtAAATGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCt < 1:551383/76‑1 (MQ=255) tAAATGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCcccc > 1:1102458/1‑76 (MQ=255) tAAATGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCcccc > 2:1489542/1‑76 (MQ=255) attGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACAGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCca < 2:781147/74‑1 (MQ=255) aaTGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCca < 2:1648390/76‑1 (MQ=255) aaTGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCca < 2:1201091/76‑1 (MQ=255) aaTGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCTcccc > 1:1013724/1‑75 (MQ=255) cGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCCCCCCCaaa > 2:812355/1‑76 (MQ=255) ggggTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCCCCCCCaaaa > 1:1039720/1‑75 (MQ=255) tACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCCCCCCCAAATTTGAc > 1:652327/1‑76 (MQ=255) accaccGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCAGGGGGGGCCCCCCCAAAATTTGag > 2:93144/1‑75 (MQ=255) accaccGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCTCCCCCAAAATTGACg > 2:932217/1‑76 (MQ=255) accaccGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCCCCCCCAAATTTTACg > 1:246717/1‑76 (MQ=255) ccaccGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCTCCCCCCAATTTGACGa > 2:857774/1‑76 (MQ=255) gAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCTCCCACAAAATTTACGATCGCt > 2:1481875/1‑76 (MQ=255) gAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCTCCCCCCAATTTTACGCACGCt > 2:1307920/1‑76 (MQ=255) gAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCTCCCCCAAATTTGACGATAGcc > 2:1103815/1‑75 (MQ=255) gAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCCCCCCCAAAATTTACGATCGcc > 2:1368677/1‑75 (MQ=255) gAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCCCCCCCAAAATTTACGATCGCt > 2:967990/1‑76 (MQ=255) gTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGgag < 1:591647/76‑1 (MQ=255) tGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCTCCCCCAAAATTGACGATCGCTGggga > 2:489573/1‑76 (MQ=255) gcttgcATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACg < 1:1105549/76‑1 (MQ=255) tgcATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCa < 2:355130/76‑1 (MQ=255) aTCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATcg < 2:1268242/76‑1 (MQ=255) aTCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCCCCCCCAAAATTGACGCTCTCTGGGGAGGACGCCACcg > 1:736080/1‑76 (MQ=255) tCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGGGGCCCCCCCAAAATTTACGAACGCTGGAGGTGACCCCCTcgc > 1:70502/1‑76 (MQ=255) tACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGaa < 2:1325749/76‑1 (MQ=255) tACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGaa < 2:191858/76‑1 (MQ=255) aCGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCTCCCCCAAAATTTACGATCGCTGGGGATGACGCCCAcgcgcaa > 2:819810/1‑73 (MQ=255) cGGCTGTTATTGGTGGCATCGGCA‑GGGGGGCCTCCCACAAATTTTACGATCGCTGGAGATGACGCCATCGCGAAGc > 2:1383832/1‑76 (MQ=255) cTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGccac > 2:477420/1‑76 (MQ=255) ttGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCacac > 2:1393538/1‑76 (MQ=255) tggtggCATCGGCA‑GGGGGGCCTCCCACCAATTTTACGATCGCTGGAGGTGACGCCATCGCGAAGCCACCCACAcc > 1:1351035/1‑76 (MQ=255) gtggCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTg < 2:1102458/76‑1 (MQ=255) gCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCgg > 1:1611869/1‑76 (MQ=255) gCATCGGCA‑GGGGGGCCTCCCACAAATTTGACGATCGCTGGAGATGATGCCATCGCGAAGCCACCCACACCTGCgg > 2:1720281/1‑76 (MQ=255) gCATCGGCA‑GGGGGGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACCCCCGccg > 2:437877/1‑74 (MQ=255) cGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTc > 2:622459/1‑76 (MQ=255) cGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTc < 1:396774/76‑1 (MQ=255) ggCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATTACGCCATCGCGAAGCCACCCACACCTGCGGTTTCa > 2:1231205/1‑76 (MQ=255) ggCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCa < 1:1365297/76‑1 (MQ=255) ggCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCa < 1:483622/76‑1 (MQ=255) a‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCAGTa > 1:131990/1‑76 (MQ=255) tGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCAGTAATTgc > 2:557097/1‑76 (MQ=255) tGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCAGTAATTgc < 1:586666/76‑1 (MQ=255) | TGTAAATGCGGGGTTACCACCGAGGGTGATTGCTTGCATCCTACGGCTGTTATTGGTGGCATCGGCA‑GGGGTGCCTCCCACAAATTTGACGATCGCTGGAGATGACGCCATCGCGAAGCCACCCACACCTGCGGTTTCAGTAATTGC > REL606/3983629‑3983775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |