Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	115.6 / NA	36	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/23);  total (13/23)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193706‑2193850                                                                          |                                                                        gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                       <  2:481692/76‑1 (MQ=255) gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                       <  1:1471363/76‑1 (MQ=255)     aTGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccccc                                                                   >  1:37658/1‑74 (MQ=255)        cATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                >  2:1103665/1‑76 (MQ=255)         aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAACCCCACCAGa                                                               >  1:356433/1‑76 (MQ=255)           ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATa                                                             >  1:732428/1‑76 (MQ=255)              gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                          <  1:1102918/76‑1 (MQ=255)                  ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  2:327095/76‑1 (MQ=255)                  ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      >  1:802903/1‑76 (MQ=255)                  ccAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      <  1:978164/76‑1 (MQ=255)                  ccAGGCGCGGCTGGGCTGGCTGTCGGAAAACAGCCCCAATAACACCAAAACGCCAATCCCCACCAGATAAAGCATg                                                      >  2:17310/1‑76 (MQ=255)                   cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                     <  2:1140141/76‑1 (MQ=255)                   cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCAAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                     >  2:1710145/1‑76 (MQ=255)                    aGGCGCGGCTGGTCTGGCTGTCGGGAATCAGCCCCAATAACAGCAAAAACCCAATCCCCACCACATAAAGCATGAc                                                    >  2:1115994/1‑76 (MQ=255)                          ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                              <  2:511300/76‑1 (MQ=255)                             tGGTCTGGCTGTCGGAAATCANCCCCAATAACAGCAAATCGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  2:272468/76‑1 (MQ=255)                                   ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:412807/76‑1 (MQ=255)                                   ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     >  2:732930/1‑76 (MQ=255)                                      tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCt                                  <  2:289448/76‑1 (MQ=255)                                       ggcGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                 <  2:1604073/74‑1 (MQ=255)                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttgc                               >  1:1411903/1‑74 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                             <  2:1536104/76‑1 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                             <  2:838973/76‑1 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                             <  1:1036794/76‑1 (MQ=255)                                               tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                         <  2:1708310/76‑1 (MQ=255)                                                cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                        >  1:1418397/1‑76 (MQ=255)                                                 aGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATg                       >  2:1333054/1‑76 (MQ=255)                                                   ccccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgc                     >  1:960648/1‑76 (MQ=255)                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                  <  1:1103665/76‑1 (MQ=255)                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                  <  2:374526/76‑1 (MQ=255)                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                 <  1:1270792/76‑1 (MQ=255)                                                         tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               <  2:735358/76‑1 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGCCGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  2:1706143/76‑1 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  1:109740/76‑1 (MQ=255)                                                               cAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCAcc         <  2:1264761/76‑1 (MQ=255)                                                                  aaCGCCAATCCCCACCAGATAAAGCACGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc      >  2:1081508/1‑76 (MQ=255)                                                                     gCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgc   >  2:1721465/1‑76 (MQ=255)                                                                      ccAATCCCCACCAGAAAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:1173340/76‑1 (MQ=255)                                                                          |                                                                        GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193706‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑