Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,015,178	A→T	D200V (GAT→GTT)	ECB_02816 →	KpsD protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,015,178	0	A	T	100.0%	200.8 / NA	53	D200V (GAT→GTT)	ECB_02816	KpsD protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/29);  total (24/29)

CGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTA  >  REL606/3015105‑3015250                                                                          |                                                                         cGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTc                                                                        >  1:528576/1‑76 (MQ=255)    aaTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCag                                                                     <  2:948455/76‑1 (MQ=255)     aTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCagn                                                                    <  1:204097/76‑2 (MQ=255)     aTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCaga                                                                    >  1:1274982/1‑76 (MQ=255)     aTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCaga                                                                    <  2:1355982/76‑1 (MQ=255)     aTCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCaga                                                                    >  1:604228/1‑76 (MQ=255)         tGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAgcgc                                                                <  2:428025/76‑1 (MQ=255)          ggTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGcgcg                                                               >  2:459643/1‑76 (MQ=255)              tGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAg                                                           >  1:1335036/1‑76 (MQ=255)              tGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAg                                                           >  1:1660187/1‑76 (MQ=255)                tATGGCGGTGTGACGTCTGATTCGTTACTCAATTGTCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGtt                                                         <  2:219727/76‑1 (MQ=255)                tATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGtt                                                         >  2:1191212/1‑76 (MQ=255)                tATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGGTGTTCCAGAGCGCGGGAggt                                                         >  2:629269/1‑74 (MQ=255)                 aTGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTa                                                        <  2:1050264/76‑1 (MQ=255)                  tGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTAc                                                       <  1:712421/76‑1 (MQ=255)                   ggCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACg                                                      <  2:884211/76‑1 (MQ=255)                        tgtgACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGat                                                 >  2:546146/1‑76 (MQ=255)                         gtgACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGata                                                <  1:812884/76‑1 (MQ=255)                              gTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATtgtg                                           >  1:1053937/1‑76 (MQ=255)                                cTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGt                                         >  1:127187/1‑76 (MQ=255)                                  gATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCa                                       >  2:638347/1‑76 (MQ=255)                                  gATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCa                                       >  1:1404949/1‑76 (MQ=255)                                  gATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCa                                       <  2:1031691/76‑1 (MQ=255)                                   aTTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCaa                                      <  2:803276/76‑1 (MQ=255)                                    ttCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAg                                     <  1:1677100/76‑1 (MQ=255)                                     tCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgc                                    <  1:1073250/76‑1 (MQ=255)                                      cGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcg                                   <  1:411713/76‑1 (MQ=255)                                      cGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcg                                   >  1:789532/1‑76 (MQ=255)                                        ttACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAgcgtg                                 <  1:1034776/76‑3 (MQ=255)                                        ttACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGcgcg                                 <  1:637932/76‑1 (MQ=255)                                        ntACTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGcgcg                                 >  1:104497/2‑76 (MQ=255)                                          aCTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGt                               >  1:1322517/1‑76 (MQ=255)                                           cTCAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTa                              <  1:1880/76‑1 (MQ=255)                                           cTCAAGTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTa                              <  2:526948/76‑1 (MQ=255)                                            tCAAGTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTaa                             <  2:1463191/76‑1 (MQ=255)                                             cAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAAc                            <  1:965795/76‑1 (MQ=255)                                             cAATTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAAc                            <  1:965778/76‑1 (MQ=255)                                               aTTATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcg                          <  2:941288/76‑1 (MQ=255)                                                ttATCTGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACcgc                         >  1:1444262/1‑76 (MQ=255)                                                     tGATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTggg                    >  1:447589/1‑74 (MQ=255)                                                      gATCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTgcgc                   >  2:502596/1‑76 (MQ=255)                                                       aTCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCt                  <  1:423351/76‑1 (MQ=255)                                                        tCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTc                 <  2:1299540/76‑1 (MQ=255)                                                        tCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTc                 <  2:203221/76‑1 (MQ=255)                                                        tCAAGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTc                 <  2:579621/76‑1 (MQ=255)                                                          aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa               <  1:19783/76‑1 (MQ=255)                                                          aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCa               >  1:49741/1‑76 (MQ=255)                                                          aaGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGAGGTAACCGCGTGCGCTCCa               >  1:510741/1‑76 (MQ=255)                                                           aGGCTGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCaa              >  1:597122/1‑76 (MQ=255)                                                               tGGCGGCGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTc          >  1:271221/1‑76 (MQ=255)                                                                ggcggcGTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTGACCGCGCGCGCTCCAACGTCa         >  2:285424/1‑76 (MQ=255)                                                                 gcggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCaa        <  2:1033949/76‑1 (MQ=255)                                                                  cggcgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAAc       >  1:333838/1‑76 (MQ=255)                                                                     cgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTg    <  2:362890/76‑1 (MQ=255)                                                                     cgTTGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTg    <  2:198115/76‑1 (MQ=255)                                                                      gttgttCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGt   >  1:570086/1‑76 (MQ=255)                                                                       ttGTTCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTa  >  1:1265280/1‑76 (MQ=255)                                                                          |                                                                         CGTAATCCTGGTCTGTATGGCGGTGTGACGTCTGATTCGTTACTCAATTATCTGATCAAGGCTGGCGGCGTTGATCCAGAGCGCGGAAGTTACGTTGATATTGTGGTCAAGCGCGGTAACCGCGTGCGCTCCAACGTCAACCTGTA  >  REL606/3015105‑3015250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑