Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,175,323	G→T	N342K (AAC→AAA)	uxaA ←	altronate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,175,323	0	G	T	100.0%	106.8 / NA	29	N342K (AAC→AAA)	uxaA	altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/11);  total (18/11)

GCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAA  >  REL606/3175252‑3175397                                                                        |                                                                           gCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTtttt                                                                        <  1:185705/76‑1 (MQ=255)   tttCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCa                                                                      >  2:1309300/1‑76 (MQ=255)    ttCTGGTTACAGCCAAGTGATTTGTCTTCAAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCAt                                                                     <  2:299362/76‑1 (MQ=255)     tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa                                                                    >  1:928609/1‑76 (MQ=255)     tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa                                                                    >  1:381424/1‑76 (MQ=255)     tCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATa                                                                    >  1:2557/1‑76 (MQ=255)       tGGGTACAGCCAAGTGATTTGTCTTCTAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGa                                                                  <  2:485219/76‑1 (MQ=255)       tGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGa                                                                  >  1:1123575/1‑76 (MQ=255)        gggTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGAt                                                                 >  2:612621/1‑76 (MQ=255)          gTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCg                                                               <  1:68811/76‑1 (MQ=255)             cAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCt                                                            >  2:27792/1‑76 (MQ=255)                ccAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGac                                                         >  1:643271/1‑75 (MQ=255)                ccAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGAt                                                         <  2:1427615/76‑1 (MQ=255)                 cAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATc                                                        >  2:1222627/1‑76 (MQ=255)                     tGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATgg                                                    >  2:1287686/1‑76 (MQ=255)                       aTTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGc                                                  >  1:612033/1‑76 (MQ=255)                         ttGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCaa                                                >  2:1363102/1‑76 (MQ=255)                          tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAAt                                               >  1:1331880/1‑76 (MQ=255)                          tGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAAt                                               >  2:1226900/1‑76 (MQ=255)                            tCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaa                                             <  1:1134349/76‑1 (MQ=255)                            tCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaa                                             <  1:980836/76‑1 (MQ=255)                             cTTCCAGCGTGGTGATACCGCCCGCTTTGTTCTCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATaaa                                            >  1:225440/1‑76 (MQ=255)                                 cAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTAc                                        >  2:802968/1‑76 (MQ=255)                                  aGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACt                                       >  2:1491250/1‑76 (MQ=255)                                  aGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCACGGGCAATAAAGTACt                                       >  1:370310/1‑76 (MQ=255)                                          gATACCGCCCGCTTTGTTCCCCGGCGATGGTTTTTCATAGATCGACTGATCATGGGCAATAAAGTACTGTTTGAAg                               <  1:855751/76‑1 (MQ=255)                                                      tttGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATg                   >  1:1603518/1‑76 (MQ=255)                                                      tttGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATg                   <  1:1514556/76‑1 (MQ=255)                                                        tGTTCCCCGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGt                 <  2:883071/76‑1 (MQ=255)                                                            ccccGGCGATGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGAcc             <  1:1463759/76‑1 (MQ=255)                                                                    aTGGTTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTc     <  2:1110725/76‑1 (MQ=255)                                                                       ggTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCaaa  <  2:1531655/76‑1 (MQ=255)                                                                        |                                                                           GCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAA  >  REL606/3175252‑3175397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑