Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,928,955	A→G	I247V (ATC→GTC)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,928,955	0	A	G	100.0%	203.2 / NA	48	I247V (ATC→GTC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

GTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGG  >  REL606/3928883‑3929027                                                                         |                                                                         gTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCg                                                                       <  2:1006054/76‑1 (MQ=255) gTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCg                                                                       >  1:66709/1‑76 (MQ=255)   aaaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCgag                                                                     >  1:990181/1‑76 (MQ=255)    aaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCgaga                                                                    <  1:721544/76‑1 (MQ=255)    aaaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCgaga                                                                    <  1:597604/76‑1 (MQ=255)     aaGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGaa                                                                   >  2:1179675/1‑76 (MQ=255)               tgttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGc                                                         <  2:934474/76‑1 (MQ=255)                gttgCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGcc                                                        >  1:1691537/1‑76 (MQ=255)                  tgCTTCTCCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGTAAATGGTGGTCGAGAAGGCCAAACGCCTg                                                      <  1:671987/76‑1 (MQ=255)                    cTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGt                                                    >  1:117322/1‑76 (MQ=255)                    cTTCTACCTTTAACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGt                                                    >  2:898039/1‑76 (MQ=255)                      tCTACCCTTGACGTACCCTCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTg                                                  <  2:1122037/76‑1 (MQ=255)                        tACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAg                                                >  2:1510586/1‑76 (MQ=255)                         aCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGc                                               <  1:513532/76‑1 (MQ=255)                           cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGcac                                             >  1:37952/1‑76 (MQ=255)                            tttGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGcaca                                            >  1:188160/1‑76 (MQ=255)                              tGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACaag                                          >  2:346282/1‑76 (MQ=255)                                aCGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACaagaa                                        >  2:1633563/1‑76 (MQ=255)                                aCGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACaagaa                                        <  2:117322/76‑1 (MQ=255)                                  gAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACaagaaag                                      >  2:588238/1‑76 (MQ=255)                                   aaCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACaagaaaga                                     <  2:495857/76‑1 (MQ=255)                                      ccGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGt                                  <  2:13847/76‑1 (MQ=255)                                       cGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGtt                                 <  2:50388/76‑1 (MQ=255)                                       cGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGtt                                 <  2:373412/76‑1 (MQ=255)                                        gCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTa                                <  1:908468/76‑1 (MQ=255)                                        gCATCTCGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTa                                <  2:879348/76‑1 (MQ=255)                                            ctcGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                            <  1:160300/76‑1 (MQ=255)                                            ctcGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                            <  1:1399596/76‑1 (MQ=255)                                            ctcGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                            >  1:370966/1‑76 (MQ=255)                                            ctcGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                            <  1:56141/76‑1 (MQ=255)                                            ctcGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCAt                            <  2:1534027/76‑1 (MQ=255)                                              cGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTc                          >  2:1168349/1‑76 (MQ=255)                                              cGCCACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTc                          >  2:192127/1‑76 (MQ=255)                                                 cACGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGc                       >  2:1489632/1‑76 (MQ=255)                                                  aCGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCt                      >  2:34939/1‑76 (MQ=255)                                                   cGTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTc                     >  2:1008195/1‑76 (MQ=255)                                                    gTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCg                    <  1:1621945/76‑1 (MQ=255)                                                    gTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCg                    <  2:773238/76‑1 (MQ=255)                                                    gTTCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCg                    <  1:236976/76‑1 (MQ=255)                                                     ttCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGa                   >  1:754739/1‑76 (MQ=255)                                                      tCAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGAc                  <  2:606619/76‑1 (MQ=255)                                                       cAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACt                 >  2:155996/1‑76 (MQ=255)                                                       cAGGTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACt                 >  2:518358/1‑76 (MQ=255)                                                         ggTTGCGGAAATGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGGCTcc               >  1:1079527/1‑76 (MQ=255)                                                                 aaaTGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCg       <  2:1115749/76‑1 (MQ=255)                                                                  gaTGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACATTATCATTCTGCTCGACTCCATCACTCGt      >  2:292592/2‑76 (MQ=255)                                                                  aaTGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGt      >  2:911585/1‑76 (MQ=255)                                                                   aTGGTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTc     >  1:205651/1‑76 (MQ=255)                                                                      gTGGTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTgg  >  1:933016/1‑76 (MQ=255)                                                                         |                                                                         GTAAAAGGTGAAGTTGTTGCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGG  >  REL606/3928883‑3929027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑