| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,168,967 | C→T | A1336V (GCC→GTC) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,168,967 | 0 | C | T | 95.9% | 167.8 / ‑0.2 | 49 | A1336V (GCC→GTC) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (22/25); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
GTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCA > REL606/4168896‑4169037 | gTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGtt < 2:546209/76‑1 (MQ=255)gTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGtt < 2:1495487/76‑1 (MQ=255) tctcTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTg < 2:313835/76‑1 (MQ=255) tctcTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTg < 2:1399081/76‑1 (MQ=255) ctctGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGc > 1:331920/1‑76 (MQ=255) ctGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCgg > 2:15198/1‑76 (MQ=255) ctGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCgg > 1:763528/1‑76 (MQ=255) tGGCAACCGTGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCggg > 2:1160747/1‑76 (MQ=255) ggCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGc > 2:811850/1‑76 (MQ=255) ggCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGc > 2:1429937/1‑76 (MQ=255) cAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCaa < 1:610068/76‑1 (MQ=255) cAACCGAGTCCTTCATCTACGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCaa > 1:254557/1‑76 (MQ=255) aaCCGAGTCCTTCATCTCCGCGGCATCGTTTCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCaaa < 1:30555/76‑1 (MQ=255) aaCCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCaaa > 1:1103940/1‑76 (MQ=255) ccGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAAcg < 2:925842/76‑1 (MQ=255) ccGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCGGACCGAAGCAGACGATGCGGGGAAAcg > 1:1681811/1‑76 (MQ=255) aGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGcga < 2:895459/76‑1 (MQ=255) aGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGcga < 2:574277/76‑1 (MQ=255) ccTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGcgacga < 2:614355/76‑1 (MQ=255) cTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGaa > 1:617021/1‑76 (MQ=255) tCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACt < 1:633582/76‑1 (MQ=255) tCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACt < 1:1460248/76‑1 (MQ=255) tCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACt < 1:9824/76‑1 (MQ=255) tCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACt < 1:186975/76‑1 (MQ=255) aTCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTgc > 1:402614/1‑76 (MQ=255) cgcgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGc > 2:1361156/1‑76 (MQ=255) cgcgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGc > 2:1208243/1‑76 (MQ=255) ctgCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCt < 2:618004/74‑1 (MQ=255) cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCt < 2:124615/76‑1 (MQ=255) cgGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCt < 2:380507/76‑1 (MQ=255) gCATCGGTCCAGGAGACCCCCCGCGGGGTGGCCGAAGCCGGCGTTGCGGGCAAACGCGACGCACCGCGCGGCCTGa > 2:241777/1‑76 (MQ=255) cATCGTTCCAGGAGCCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGaa < 1:1307463/76‑1 (MQ=255) cATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGaa > 1:1445985/1‑76 (MQ=255) ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAgaga < 2:1134737/76‑1 (MQ=255) ttCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAgaga < 2:833461/76‑1 (MQ=255) tgccAGGAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAgaga < 2:1092604/74‑1 (MQ=255) tCCAGGAGACCACTCGCGTGCTGACCGAAGCAGTCTTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGaa < 2:1115787/76‑1 (MQ=255) cAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACg < 1:663636/76‑1 (MQ=255) ggAGACCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGtt < 1:643888/76‑1 (MQ=255) gagaCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTa < 1:1281314/76‑1 (MQ=255) agaCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTAt > 2:1014078/1‑76 (MQ=255) agaCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTAt > 2:15103/1‑76 (MQ=255) agaCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTAt < 2:1563690/76‑1 (MQ=255) gaCCACTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATc < 2:670502/76‑1 (MQ=255) cTCGCGTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTggg > 1:86433/1‑76 (MQ=255) gcgTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcg < 2:1192094/76‑1 (MQ=255) cgTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcgt > 2:205006/1‑76 (MQ=255) gTGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGgtcgtc < 2:632176/76‑1 (MQ=255) tGCTGACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCt > 1:1439455/1‑76 (MQ=255) gACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGGTc > 1:981607/1‑76 (MQ=255) gACCGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATc > 1:515584/1‑76 (MQ=255) ccGAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATccc > 1:445854/1‑76 (MQ=255) cGAAGCAGTCGGTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCg < 1:14430/76‑1 (MQ=255) gAAGCAGTCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCgg > 1:1051038/1‑76 (MQ=255) agcagtCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCa < 1:451771/76‑1 (MQ=255) | GTCTCTGGCAACCGAGTCCTTCATCTCCGCGGCATCGTTCCAGGAGACCACTCGCGTGCTGACCGAAGCAGCCGTTGCGGGCAAACGCGACGAACTGCGCGGCCTGAAAGAGAACGTTATCGTGGGTCGTCTGATCCCGGCA > REL606/4168896‑4169037 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |