Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	447,351	G→A	intergenic (‑123/‑95)	tesB ← / → ybaY	acyl‑CoA thioesterase II/predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	447,351	0	G	A	100.0%	210.4 / NA	52	intergenic (‑123/‑95)	tesB/ybaY	acyl‑CoA thioesterase II/predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/26);  total (26/26)

ATAACGCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTGATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGT  >  REL606/447278‑447422                                                                          |                                                                        ataACGCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAAt                                                                       >  1:1389509/1‑76 (MQ=255)  taACGCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAAtg                                                                      >  1:996626/1‑76 (MQ=255)  taACGCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAAtg                                                                      <  2:1051388/76‑1 (MQ=255)      gCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAAtgtgtg                                                                  <  1:1025863/76‑1 (MQ=255)      gCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAAtgtgtg                                                                  <  1:991331/76‑1 (MQ=255)       cAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGc                                                                 >  1:647696/1‑76 (MQ=255)        aaaTTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGcc                                                                >  2:682901/1‑76 (MQ=255)             tGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACt                                                           <  1:1200473/76‑1 (MQ=255)              gCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACtt                                                          >  1:492884/1‑76 (MQ=255)              gCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACtt                                                          >  2:1103831/1‑76 (MQ=255)              gCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACtt                                                          >  2:359188/1‑76 (MQ=255)               ccACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTa                                                         >  1:1217049/1‑76 (MQ=255)                 aCTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTAtt                                                       <  2:1402734/76‑1 (MQ=255)                 aCTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTAtt                                                       <  1:764700/76‑1 (MQ=255)                  cTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTg                                                      <  2:1471574/76‑1 (MQ=255)                  cTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTg                                                      <  1:779996/76‑1 (MQ=255)                  cTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTg                                                      <  1:1196831/76‑1 (MQ=255)                   tGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGa                                                     >  2:1466641/1‑76 (MQ=255)                       ccGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGtt                                                 <  2:408504/76‑1 (MQ=255)                       ccGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGtt                                                 >  1:1429455/1‑76 (MQ=255)                        cGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTa                                                >  1:216984/1‑76 (MQ=255)                        cGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTa                                                >  2:108850/1‑76 (MQ=255)                         gATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTAc                                               >  2:983240/1‑76 (MQ=255)                         gATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTAc                                               >  2:489197/1‑76 (MQ=255)                            gggTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTAt                                            <  1:1656090/76‑1 (MQ=255)                             ggTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTAtt                                           <  1:616566/76‑1 (MQ=255)                             ggTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTAtt                                           <  1:69264/76‑1 (MQ=255)                               tGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTAtttt                                         >  1:860739/1‑76 (MQ=255)                               tGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTAtttt                                         >  2:5997/1‑76 (MQ=255)                                  aataatCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGtt                                      <  2:1331718/76‑1 (MQ=255)                                    taatCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTaa                                    >  1:1339369/1‑76 (MQ=255)                                    taatCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTaa                                    <  2:53191/76‑1 (MQ=255)                                     aatCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAAc                                   <  1:1646081/76‑1 (MQ=255)                                       tCTGAATTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCa                                 >  2:159247/1‑76 (MQ=255)                                            aTTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCtt                            <  1:565097/76‑1 (MQ=255)                                            aTTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCtt                            <  2:633766/76‑1 (MQ=255)                                            aTTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCtt                            <  1:1570580/76‑1 (MQ=255)                                            aTTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCtt                            <  1:370550/76‑1 (MQ=255)                                            aTTACGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCtt                            <  2:324853/76‑1 (MQ=255)                                               aCGGGCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCg                         <  2:33868/76‑1 (MQ=255)                                                 gggCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGc                       <  2:1600166/76‑1 (MQ=255)                                                   gCTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGa                     <  2:774964/76‑1 (MQ=255)                                                    cTAATTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAg                    >  1:1213228/1‑76 (MQ=255)                                                      aaTTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGa                  >  2:419850/1‑76 (MQ=255)                                                      aaTTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGa                  >  2:575990/1‑76 (MQ=255)                                                      aaTTACAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGa                  >  2:746976/1‑76 (MQ=255)                                                          aCAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGt              >  1:1283345/1‑76 (MQ=255)                                                           cAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGtt             >  2:1692110/1‑76 (MQ=255)                                                           cAGGCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGtt             <  1:426624/76‑1 (MQ=255)                                                             ggCAGAAATGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAg           <  2:1416273/76‑1 (MQ=255)                                                                  aaaTGCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGc      >  1:1574083/1‑76 (MQ=255)                                                                      gCGTAATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGt  >  1:870156/1‑76 (MQ=255)                                                                          |                                                                        ATAACGCAAATTTGCCACTGGTCCGATGGGTGCAATAATCTGAATTACGGGCTAATTACAGGCAGAAATGCGTGATGTGTGCCACACTTATTGACGTTACTATTTTGTTAACCACTCTTCCGGCGAGGAAAGTTAGCCCGCTGGT  >  REL606/447278‑447422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑