Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,031,943	C→T	P285P (CCG→CCA)	manB ←	Phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,031,943	0	C	T	100.0%	62.0 / NA	19	P285P (CCG→CCA)	manB	Phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/11);  total (8/11)

CGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGCGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCG  >  REL606/2031870‑2031979                                                                          |                                     cGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTgg                                    <  2:256442/76‑1 (MQ=255)       cGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTg                              <  2:764860/76‑1 (MQ=255)           tttCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGgatg                          <  1:1236849/76‑1 (MQ=255)            ttCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGgatga                         <  1:855600/76‑1 (MQ=255)               gACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCt                      >  1:1691462/1‑76 (MQ=255)               gACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCt                      >  1:563605/1‑76 (MQ=255)                 cATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTc                    <  2:43784/76‑1 (MQ=255)                  aTCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTcg                   <  2:845337/76‑1 (MQ=255)                    cACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTcgcg                 >  2:546638/1‑76 (MQ=255)                      ccGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGGGGGTGATCTTCGCGcc               >  2:1643426/1‑76 (MQ=17)                       cGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCg              >  2:1089160/1‑76 (MQ=255)                        ggAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCgg             >  2:1114561/1‑76 (MQ=255)                        ggAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCCCCgg             >  2:1622598/1‑76 (MQ=255)                         gAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCggg            >  2:434876/1‑76 (MQ=255)                         gAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCggg            <  2:909376/76‑1 (MQ=255)                          aGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGa           >  1:1553050/1‑76 (MQ=255)                            tGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGAtt         <  2:1432378/76‑1 (MQ=255)                             gccgccCGCGGCGGTCACCACATCAAAGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGAttt        >  2:592451/1‑76 (MQ=14)                             gccgccCGCGGCGGGCACCACATCACAGGTGATCCAGGAACGACGTGGATCGTGGCTGATCTTCGCGCCGGGCttt        >  2:22207/1‑76 (MQ=14)                                   cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg  <  1:1147145/76‑1 (MQ=25)                                   cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg  <  1:535819/76‑1 (MQ=25)                                   cgcgGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCg  <  1:535801/76‑1 (MQ=25)                                                                          |                                     CGTGTCCGGTTTTCGACATCACCGGAGTGCCGCCCGCGGCGGTCACCACATCAACGGTGTTCCAGGAGAGACGCGGATCGTGGATGATCTTCGCGCCGGGATTTTTTTCG  >  REL606/2031870‑2031979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑