Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,032,210	T→A	A196A (GCA→GCT)	manB ←	Phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,032,210	0	T	A	100.0%	51.9 / NA	16	A196A (GCA→GCT)	manB	Phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/7);  total (9/7)

AATTGCCGTCCGGCGTGTTGTGTACTTTGATTAATTCCACCGGTGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTCCCGG  >  REL606/2032167‑2032274                                            |                                                                 aaTTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGc                                  <  2:1164912/76‑1 (MQ=255) aaTTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGc                                  <  2:805857/76‑1 (MQ=255) aaTTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGc                                  <  2:1664919/76‑1 (MQ=255)     gCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTcc                              >  1:654112/1‑76 (MQ=255)        gTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTccacc                           <  1:1515173/76‑1 (MQ=255)              cGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGc                     >  1:846659/1‑76 (MQ=255)                tgttgtGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGccc                   >  1:1478137/1‑76 (MQ=255)                  ttgtGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCccg                 >  2:1379726/1‑76 (MQ=255)                    gtgcACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCccgcc               <  1:1166835/76‑1 (MQ=255)                           ttGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCAt        >  2:493837/1‑76 (MQ=255)                            tGATTAATTCCCCCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCCCCACCGGCCCCGCCGCGCCAtt       <  2:717683/76‑1 (MQ=17)                            tGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCAtt       >  1:203043/1‑76 (MQ=255)                               ttAATTCCCCCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTccc    <  2:1373144/76‑1 (MQ=255)                               ttAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTccc    <  1:636490/76‑1 (MQ=255)                               ttAATTCCACCGGACCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCCCGCCATTccc    >  2:794645/1‑76 (MQ=17)                                tAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTCCCg   >  1:851187/1‑76 (MQ=255)                                tAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTCCCg   >  1:123327/1‑76 (MQ=255)                                 aaTTCCCCCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGCCCACCCCCGGCCCCGCCGCGCCATTCCCgg  <  2:1343187/76‑1 (MQ=17)                                 aaTTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTCCCgg  <  2:442395/76‑1 (MQ=255)                                            |                                                                 AATTGCCGTCCGGCGTGTTGTGTACTTTGATTAATTCCACCGGTGCGCCGAGGGCTTTAAAGCGGGCTTCAATGGCGTCCACCACCGGCCCCGCCGCGCCATTCCCGG  >  REL606/2032167‑2032274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑