Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,055,515	G→T	N194K (AAC→AAA)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,055,515	0	G	T	93.8%	45.6 / ‑2.0	16	N194K (AAC→AAA)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (7/8);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCG  >  REL606/2055442‑2055560                                                                          |                                              tAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCttt                                             >  1:1316461/1‑76 (MQ=21)  aGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTa                                            <  1:837282/76‑1 (MQ=21)     aaTACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAg                                         >  1:1419726/1‑76 (MQ=21)       tACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCg                                       >  2:1249168/1‑76 (MQ=21)         ccGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCggg                                     >  1:728035/1‑76 (MQ=21)         ccGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCggg                                     >  1:807131/1‑76 (MQ=21)            ttGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCtt                                  <  2:508806/76‑1 (MQ=255)            ttGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCtt                                  <  2:10786/76‑1 (MQ=255)             tGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTc                                 >  2:823410/1‑76 (MQ=255)             tGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTc                                 >  2:1454338/1‑76 (MQ=255)              ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:753562/76‑2 (MQ=255)              ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:590630/76‑2 (MQ=255)              ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:243554/76‑2 (MQ=255)              ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:229518/76‑2 (MQ=255)              ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                >  1:1666040/1‑75 (MQ=255)                ggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAAt                              <  1:1143633/76‑1 (MQ=255)                                            gttGATTAATTCCGCCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCg  <  2:657806/75‑1 (MQ=255)                                                                          |                                              TAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCG  >  REL606/2055442‑2055560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑