| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,995,676 | (C)8→7 | intergenic (+103/+351) | yeeN → / ← asnW | hypothetical protein/tRNA‑Asn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,995,669 | 0 | C | . | 95.3% | 291.3 / 9.7 | 64 | intergenic (+96/+358) | yeeN/asnW | hypothetical protein/tRNA‑Asn |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base . (24/37); total (25/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAG > REL606/1995594‑1995740 | tCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAc < 1:343618/76‑1 (MQ=255) tACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccccc < 1:1047223/76‑1 (MQ=255) cGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAccccccc < 2:500775/76‑1 (MQ=255) cAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccccgtt > 2:960558/1‑73 (MQ=255) cAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATAcccccccgtt > 2:1107433/1‑73 (MQ=255) aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc < 2:1596659/76‑1 (MQ=255) aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc < 1:794792/76‑1 (MQ=255) aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc < 1:794799/76‑1 (MQ=255) aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc < 2:1193953/76‑1 (MQ=255) aTTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTccc > 2:1359979/1‑76 (MQ=255) tttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCa < 1:1742517/76‑1 (MQ=255) ttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCag < 2:1690837/76‑1 (MQ=255) ttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCag > 1:555583/1‑76 (MQ=255) cTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCagag > 1:1228131/1‑76 (MQ=255) tttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAgaga < 2:771962/76‑1 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGaa < 1:844320/76‑1 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGaa < 2:254051/76‑1 (MQ=255) ttAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGaa < 1:1418431/76‑1 (MQ=255) tAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGaa > 1:123900/1‑76 (MQ=255) aaTCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaat < 2:1555167/76‑1 (MQ=255) aaTCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaat > 1:534651/1‑76 (MQ=255) aTCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaata < 1:1132535/76‑1 (MQ=255) aTCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaata < 1:1206254/76‑1 (MQ=255) aTCTCATATAATTCTTATAAAAAATATAATATTCAAATCGTCATATTGATTATA‑CCCCCCCGTTCCCCGAGAaata > 1:470526/1‑76 (MQ=255) tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAcataa > 1:1010511/1‑76 (MQ=255) tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaataa > 1:1010532/1‑76 (MQ=255) tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAaataa < 1:1091152/76‑1 (MQ=255) atatAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatt < 2:1121211/76‑1 (MQ=255) atatAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatt > 2:1054211/1‑76 (MQ=255) tataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtattt < 1:304805/76‑1 (MQ=255) ataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCC‑TTCCCAGAGAAATAAtattta < 2:1330075/76‑1 (MQ=255) ataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtattta < 2:1330067/76‑1 (MQ=255) taATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatttat > 2:887368/1‑76 (MQ=255) aaTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatttatt > 1:709033/1‑76 (MQ=255) aaTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatttatt < 2:493297/76‑1 (MQ=255) aaTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAAtatttatt < 2:493294/76‑1 (MQ=255) aTTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATttatta > 1:977970/1‑76 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTaaa < 1:430037/76‑1 (MQ=255) tCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTaaa < 2:542172/76‑1 (MQ=255) tataAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAAtt > 2:671173/1‑76 (MQ=255) aaaaaTATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg < 2:479950/76‑1 (MQ=255) aaaaaTATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAg < 1:1450628/76‑1 (MQ=255) atataatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttct < 2:1434902/76‑1 (MQ=255) atataatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttct < 2:1222366/76‑1 (MQ=255) ataatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGttcttc > 1:664916/1‑76 (MQ=255) aatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt < 1:973743/76‑1 (MQ=255) aatatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt > 1:853623/1‑76 (MQ=255) atatTCAACTCGTCATATTGATTATA‑CTCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttt < 1:473870/76‑1 (MQ=255) atatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttt < 2:1081327/76‑1 (MQ=255) atatTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttt < 2:750271/76‑1 (MQ=255) atTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt > 1:1503353/1‑76 (MQ=255) tCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt < 1:637446/76‑1 (MQ=255) cAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTg > 1:1457550/1‑76 (MQ=255) cAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGCTCTTCTTTTTCTg > 1:1457543/1‑76 (MQ=255) cTCGTCATATTGATTATA‑CCCCCCCGTTCCCCGAGAAATAATATTTATTAAAATTTCAGTTCTTCTTTTTTTTAtt > 1:437815/1‑76 (MQ=255) cTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGAtt > 1:218875/1‑76 (MQ=255) tCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTTTTTTTCTGATTa > 1:1505114/1‑76 (MQ=255) tCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa < 1:615687/76‑1 (MQ=255) tCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTa > 1:1545506/1‑76 (MQ=255) cGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc > 1:1396833/1‑76 (MQ=255) cGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc > 1:513679/1‑76 (MQ=255) gTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc < 2:1548053/76‑1 (MQ=255) gTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACa < 1:911361/76‑1 (MQ=255) tCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAg < 1:404305/76‑1 (MQ=255) atatTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGaa < 1:978722/76‑1 (MQ=255) tatTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAg < 2:587901/76‑1 (MQ=255) gATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa < 2:1115479/76‑1 (MQ=255) gATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCaa < 2:1115472/76‑1 (MQ=255) ttATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAg > 2:693447/1‑76 (MQ=255) | TCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAG > REL606/1995594‑1995740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |