| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,965 | 1 | . | T | 98.1% | 202.4 / ‑2.7 | 52 | intergenic (‑166/‑157) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (28/23); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911892‑3912034 | aaTGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑tt < 1:1545921/76‑1 (MQ=255) atgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt > 1:1280002/1‑76 (MQ=255) atgatgCTCACAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt < 1:854612/76‑1 (MQ=255) tgatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑t > 1:218224/1‑76 (MQ=255) gatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑tt < 1:990370/76‑1 (MQ=255) gatgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑tt < 2:1201231/76‑1 (MQ=255) atgCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑ttt > 1:483995/1‑76 (MQ=255) gCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑ttttt < 2:402614/76‑2 (MQ=255) cTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑tttttc < 2:1440809/76‑3 (MQ=255) tCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑ttt‑tttttca < 1:467644/76‑4 (MQ=255) cAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTtcactc < 2:426267/76‑1 (MQ=255) aaaaaTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTtcactca > 2:133164/1‑76 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtat > 1:216367/1‑76 (MQ=255) aTGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtat > 1:270611/1‑76 (MQ=255) tGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtatt < 1:65783/76‑1 (MQ=255) cAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtatttt < 2:1402705/76‑1 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtattttatt > 1:212464/1‑76 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtattttatt > 1:422690/1‑76 (MQ=255) cGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtattttatt > 2:681693/1‑76 (MQ=255) ggACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACtattttattt < 1:344628/76‑1 (MQ=255) gACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTattttatttt > 2:221383/1‑76 (MQ=255) aCAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTAttttt < 1:363228/76‑1 (MQ=255) cAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTa > 1:473499/1‑76 (MQ=255) ggATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTaaaaa < 1:246402/76‑1 (MQ=255) gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAaacaa < 1:813074/76‑1 (MQ=255) cTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAAcaacaa > 2:1752318/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 1:948585/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAAt > 1:1454830/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat > 1:883792/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTtat < 2:484423/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATAtt > 1:1167211/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg < 2:1156786/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg > 2:666763/1‑76 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg < 2:10901/76‑1 (MQ=255) aGGGAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg < 1:268364/76‑1 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa < 1:1349750/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt < 1:983107/76‑1 (MQ=255) caacaTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatta > 1:278867/1‑76 (MQ=255) acaTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt > 1:1019121/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta < 1:529457/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa > 1:922301/1‑76 (MQ=255) aTTCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa > 1:160268/1‑76 (MQ=255) ttCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa > 2:721774/1‑76 (MQ=255) ttCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa > 1:342233/1‑76 (MQ=255) ttCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:199672/76‑1 (MQ=255) ttCATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa < 1:1429276/76‑1 (MQ=255) cATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAGAAAACAACAATTTATATTGAAATTATTAAACg > 1:316747/1‑76 (MQ=255) cATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg > 1:1441424/1‑76 (MQ=255) cATACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACg > 1:1115179/1‑76 (MQ=255) tACTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGca > 2:528356/1‑76 (MQ=255) aCTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 1:1550740/1‑76 (MQ=255) aCTGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTNATATTGAAATTATTAAACGcat < 2:244044/76‑1 (MQ=255) tGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca < 2:843755/76‑1 (MQ=255) tGAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatca < 2:1108150/76‑1 (MQ=255) gAAATTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat > 2:264186/1‑76 (MQ=255) aaaTTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:473499/76‑1 (MQ=255) aaaTTGAA‑TTTCTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa > 2:390780/1‑76 (MQ=255) aaTTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 2:617699/76‑1 (MQ=255) aaTTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:636352/76‑1 (MQ=255) gttGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:477513/2‑76 (MQ=255) aTTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:527373/76‑1 (MQ=255) aTTGAATTTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:477499/1‑76 (MQ=255) | AATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAA‑TTT‑TTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAA > REL606/3911892‑3912034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |