BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,779,616	G→A	S74S (TCC→TCT)	thrS ←	threonyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,779,616	0	G	A	100.0%	258.5 / NA	65	S74S (TCC→TCT)	thrS	threonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/31); total (34/31)

CAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATC  >  REL606/1779543‑1779688

cAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGa                                                                        >  1:729932/1‑76 (MQ=255)

 aaTAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAagag                                                                       >  2:22250/1‑76 (MQ=255)

   tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg                                                                     >  2:1767328/1‑76 (MQ=255)

   tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg                                                                     >  2:774246/1‑76 (MQ=255)

   tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg                                                                     >  2:39833/1‑76 (MQ=255)

    aaCCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGa                                                                    >  1:656489/1‑76 (MQ=255)

      ccGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACg                                                                  <  2:333159/76‑1 (MQ=255)

       cGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGa                                                                 >  1:1332424/1‑76 (MQ=255)

       cGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGa                                                                 >  2:621562/1‑76 (MQ=255)

         ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAAt                                                               <  2:1560413/76‑1 (MQ=255)

          gCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATg                                                              <  2:1613044/76‑1 (MQ=255)

            cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt                                                            >  1:1764566/1‑76 (MQ=255)

            cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt                                                            <  1:338931/76‑1 (MQ=255)

            cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt                                                            <  1:930140/76‑1 (MQ=255)

             gATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAtc                                                           >  1:1430973/1‑76 (MQ=255)

                tGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTcc                                                        >  2:1056545/1‑76 (MQ=255)

                 gCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCa                                                       <  1:106042/76‑1 (MQ=255)

                  ccATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAg                                                      >  1:1252753/1‑76 (MQ=255)

                   cATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGAGACGAATGATCTCCAGa                                                     <  2:423385/76‑1 (MQ=255)

                    aTTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGAc                                                    >  1:1229785/1‑76 (MQ=255)

                      tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct                                                  >  1:273806/1‑76 (MQ=255)

                      tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct                                                  >  1:1657731/1‑76 (MQ=255)

                      tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct                                                  >  1:759428/1‑76 (MQ=255)

                       ttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACctt                                                 <  2:932169/76‑1 (MQ=255)

                        tGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttc                                                <  2:1385816/76‑1 (MQ=255)

                          gTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttctt                                              <  2:579788/76‑1 (MQ=255)

                          gTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttctt                                              <  1:445908/76‑1 (MQ=255)

                           tATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCttcttc                                             >  1:1583531/1‑76 (MQ=255)

                            aTGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCg                                            <  1:1620238/76‑1 (MQ=255)

                             tGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGt                                           <  1:39318/76‑1 (MQ=255)

                               cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt                                         <  2:1004484/76‑1 (MQ=255)

                               cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt                                         >  2:345126/1‑76 (MQ=255)

                               cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt                                         >  1:351720/1‑76 (MQ=255)

                               cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCGTCTTCGTCt                                         <  2:1127104/76‑1 (MQ=255)

                                 gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt                                       <  1:113379/76‑1 (MQ=255)

                                 gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt                                       <  1:942334/76‑1 (MQ=255)

                                 gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt                                       <  2:404641/76‑1 (MQ=255)

                                       gttgttTAATCGCGTGCTCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGt                                 >  1:1344667/1‑76 (MQ=255)

                                       gttgttTAATCGCGTGCTCTAACAGGCGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCggg                                 >  1:1340812/1‑75 (MQ=255)

                                       gttgttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGt                                 <  2:1631058/76‑1 (MQ=255)

                                        ttgttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTa                                <  2:1641511/76‑1 (MQ=255)

                                          gttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAAt                              <  2:506758/76‑1 (MQ=255)

                                              aaTCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATc                          >  2:1020736/1‑76 (MQ=255)

                                                tCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGa                        >  2:427444/1‑76 (MQ=255)

                                                 cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc                       <  1:1368462/76‑1 (MQ=255)

                                                 cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc                       >  1:889340/1‑76 (MQ=255)

                                                 cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc                       <  2:480035/76‑1 (MQ=255)

                                                 cgcgTGCCCTAACAGGTGCGCACAAGAGGGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc                       >  2:215622/1‑76 (MQ=255)

                                                     tGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGtt                   >  1:1095391/1‑76 (MQ=255)

                                                     tGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGtt                   >  1:430258/1‑76 (MQ=255)

                                                      gCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtg                  >  2:1722868/1‑76 (MQ=255)

                                                      gCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtg                  >  2:460069/1‑76 (MQ=255)

                                                       cccTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtgt                 <  1:954166/76‑1 (MQ=255)

                                                        ccTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtgtg                >  2:467000/1‑76 (MQ=255)

                                                         cTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGc               >  1:1246205/1‑76 (MQ=255)

                                                           aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt             <  1:1238595/76‑1 (MQ=255)

                                                           aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt             >  2:386495/1‑76 (MQ=255)

                                                           aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt             >  1:242885/1‑76 (MQ=255)

                                                            aCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtc            <  2:1397124/76‑1 (MQ=255)

                                                            aCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtc            <  2:1260490/76‑1 (MQ=255)

                                                                gTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGttt        <  2:1083403/76‑1 (MQ=255)

                                                                   cgcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCa     <  1:547358/76‑1 (MQ=255)

                                                                   cgcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCa     <  1:981109/76‑1 (MQ=255)

                                                                    gcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCaa    <  1:952358/76‑1 (MQ=255)

                                                                      acaAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATc  >  1:23975/1‑76 (MQ=255)

                                                                      acaAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATc  >  1:1649873/1‑76 (MQ=255)

CAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATC  >  REL606/1779543‑1779688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`