Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,213,269	C→A	K95N (AAG→AAT)	fruK ←	1‑phosphofructokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,213,269	0	C	A	100.0%	169.0 / NA	45	K95N (AAG→AAT)	fruK	1‑phosphofructokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/16);  total (29/16)

GTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGC  >  REL606/2213196‑2213341                                                                          |                                                                         gTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCTGCtt                                                                        >  1:844698/1‑76 (MQ=255) gTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGAtt                                                                        <  1:674560/76‑1 (MQ=255)  ttCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTa                                                                       >  1:1340075/1‑76 (MQ=255)  ttCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTa                                                                       >  2:1487589/1‑76 (MQ=255)  ttCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTa                                                                       <  1:662583/76‑1 (MQ=255)   tCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTaa                                                                      >  1:486254/1‑76 (MQ=255)      cAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGt                                                                   >  1:1413732/1‑76 (MQ=255)         tCGGCGGGGGTGACTTCAAATCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTaa                                                                <  1:462410/76‑1 (MQ=255)             cggGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCg                                                            <  1:1112178/76‑1 (MQ=255)                 ggTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTg                                                        <  1:411677/76‑1 (MQ=255)                   tGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTgcg                                                      >  1:801184/1‑76 (MQ=255)                    gACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTgcgc                                                     >  2:85280/1‑76 (MQ=255)                     aCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGcc                                                    >  2:615061/1‑76 (MQ=255)                     aCTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTACGTCAGATTAACGTTAATTCGAGTGCGcc                                                    >  2:306371/1‑76 (MQ=255)                         cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTg                                                >  2:366956/1‑76 (MQ=255)                         cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTg                                                >  2:1242179/1‑76 (MQ=255)                         cAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTg                                                >  1:947461/1‑76 (MQ=255)                          aaaaCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGt                                               >  2:796163/1‑76 (MQ=255)                           aaaCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTa                                              >  2:267897/1‑76 (MQ=255)                            aaCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTAc                                             >  2:1483212/1‑76 (MQ=255)                            aaCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTAc                                             <  1:936580/76‑1 (MQ=255)                             aCCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACa                                            >  1:213597/1‑76 (MQ=255)                                cGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAAcc                                         >  1:1547203/1‑76 (MQ=255)                                 gagaAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAAccn                                        <  1:1365448/76‑2 (MQ=255)                                    aaGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGa                                     <  2:1359977/76‑1 (MQ=255)                                     aGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGaa                                    <  1:1412366/76‑1 (MQ=255)                                     aGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGaa                                    >  1:522431/1‑76 (MQ=255)                                         gAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACgg                                >  2:220357/1‑76 (MQ=255)                                         gAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACgg                                <  1:958331/76‑1 (MQ=255)                                         gAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACgg                                >  1:1559719/1‑76 (MQ=255)                                         gAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACgg                                >  1:178576/1‑76 (MQ=255)                                            gTCGGTCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTg                             >  1:1762182/1‑76 (MQ=255)                                                 tCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAAt                        <  2:669678/76‑1 (MQ=255)                                                 tCACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAAt                        >  2:348026/1‑76 (MQ=255)                                                  cACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATg                       >  2:1272021/1‑76 (MQ=255)                                                  cACTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATg                       >  2:1546559/1‑76 (MQ=255)                                                   aCTTCGCCGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGc                      >  2:1571213/1‑76 (MQ=255)                                                          cGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTc               <  1:1715681/76‑1 (MQ=255)                                                          cGTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTc               <  1:1741736/76‑1 (MQ=255)                                                           gTCTTTTTCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGTCCAGCTCg              <  2:939304/76‑1 (MQ=255)                                                               ttttCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGa          >  2:1755423/1‑76 (MQ=255)                                                               ttttCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGa          >  1:1748896/1‑76 (MQ=255)                                                               ttttCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGa          >  2:810283/1‑76 (MQ=255)                                                                  tCCGTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACa       >  1:146214/1‑76 (MQ=255)                                                                     gTCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGtt    <  1:739370/76‑1 (MQ=255)                                                                      tCAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTg   <  2:973164/76‑1 (MQ=255)                                                                       cAGATTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGc  >  1:1496871/1‑76 (MQ=255)                                                                          |                                                                         GTTCCCAGTCGGCGGGGGTGACTTCAAAACCCGAGAAGTTGAAGTCGGTCACTTCGCCGTCTTTTTCCGTCAGCTTAACGTTAATTCGAGTGCGCCCCTGTACAACCTGGAAACGGTTGGCAATGCCCAGCTCGCTGAACAGTTGC  >  REL606/2213196‑2213341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑