| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,250,438 | A→C | S271A (TCA→GCA) | infB ← | translation initiation factor IF‑2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,250,438 | 0 | A | C | 98.4% | 255.1 / ‑3.0 | 62 | S271A (TCA→GCA) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (32/29); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01 | |||||||||||
GCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAC > REL606/3250366‑3250510 | gCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTc < 2:403854/76‑1 (MQ=255) cGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCa < 2:1680075/76‑1 (MQ=255) cGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCa < 2:11544/76‑1 (MQ=255) gaagaaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAg < 1:969664/76‑1 (MQ=255) aagaaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGc < 1:34504/76‑1 (MQ=255) aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 2:813396/1‑76 (MQ=255) aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 2:1036332/1‑76 (MQ=255) aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 1:1676521/1‑76 (MQ=255) aCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtgt > 1:1049269/1‑76 (MQ=255) ccTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtt < 1:7400/76‑1 (MQ=255) cTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgttt < 2:1490216/76‑1 (MQ=255) tACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtttgtt < 2:1736279/76‑1 (MQ=255) tACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtttgtt < 2:1324966/76‑1 (MQ=255) cGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGc < 2:639886/76‑1 (MQ=255) gTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGcc < 2:365120/76‑1 (MQ=255) tttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCtt > 1:357868/1‑76 (MQ=255) ttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCttt > 1:1186147/1‑76 (MQ=255) ttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCttt < 2:1203945/76‑1 (MQ=255) ccGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttct < 1:717151/76‑1 (MQ=255) ccGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttct > 1:846273/1‑76 (MQ=255) cGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttctt < 1:1080501/76‑1 (MQ=255) gCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttcttc < 2:980136/76‑1 (MQ=255) tttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGa > 2:518980/1‑76 (MQ=255) cttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGa < 2:346230/75‑1 (MQ=255) ttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAc > 1:216828/1‑76 (MQ=255) ttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAc > 1:1610569/1‑76 (MQ=255) tACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcg < 1:669324/76‑1 (MQ=255) tACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcg < 2:392738/76‑1 (MQ=255) aCCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgc > 1:1470707/1‑76 (MQ=255) aCCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgc > 1:827706/1‑76 (MQ=255) ccGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg > 1:1194174/1‑76 (MQ=255) cGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACgcgc > 1:727856/1‑76 (MQ=255) cGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACgcgc > 1:25328/1‑76 (MQ=255) ccACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCgctg > 2:1264741/1‑76 (MQ=255) ccACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCgctg > 1:1006642/1‑76 (MQ=255) gTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt < 2:1181659/76‑1 (MQ=255) gTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt < 2:1357984/76‑1 (MQ=255) tACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc < 2:645890/76‑1 (MQ=255) tACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc < 2:1364982/76‑1 (MQ=255) cgttgctgcGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc > 2:1403384/4‑76 (MQ=255) aCTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCNTTCTTCGGACGCGCTGCTTTcg < 2:246472/76‑1 (MQ=255) ctgctgCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTcgc < 2:1613717/76‑1 (MQ=255) ctgcGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGtt < 2:622822/76‑1 (MQ=255) gcgcGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTAc > 2:698391/1‑76 (MQ=255) gTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcc > 1:121953/1‑76 (MQ=255) tGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcca > 1:1043443/1‑76 (MQ=255) gCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccac > 1:803242/1‑76 (MQ=255) gCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccac > 1:365640/1‑76 (MQ=255) cttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcc > 2:992057/1‑76 (MQ=255) cttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcc > 1:1311398/1‑76 (MQ=255) ttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcca < 1:373077/76‑1 (MQ=255) tcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggccac < 2:900256/76‑1 (MQ=255) cACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTCCGCGTTACGAccacggccacg > 1:958476/1‑76 (MQ=255) cACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTAAGAccacggccacg > 1:563880/1‑76 (MQ=255) aGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCt < 1:61435/76‑1 (MQ=255) aGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCt > 1:811025/1‑76 (MQ=255) gCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGgccgccct > 1:992312/1‑74 (MQ=255) gCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCtt < 2:1592045/76‑1 (MQ=255) cTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTc > 1:772137/1‑76 (MQ=255) cTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTc > 1:1352302/1‑76 (MQ=255) tttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGa > 2:369997/1‑76 (MQ=255) ttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAc > 2:742413/1‑76 (MQ=255) ttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCCCCGAc > 1:616602/1‑76 (MQ=255) | GCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAC > REL606/3250366‑3250510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |