| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,332,148 | (T)6→7 | intergenic (+131/‑79) | topA → / → cysB | DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,332,142 | 1 | . | T | 97.3% | 134.2 / ‑3.0 | 37 | intergenic (+125/‑85) | topA/cysB | DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (19/17); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
TTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑TTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAA > REL606/1332067‑1332212 | ttttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAc‑c > 1:1506734/1‑76 (MQ=255) ttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑tt > 2:1182805/1‑76 (MQ=255) ttttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑tt > 1:1124259/1‑76 (MQ=255) ttATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑tttt > 2:318751/1‑76 (MQ=255) aTTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑tttttt < 1:1460355/76‑1 (MQ=255) aTTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑tttttt > 1:169521/1‑76 (MQ=255) tattTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTaa < 1:27521/76‑1 (MQ=255) ttttttCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAAt < 2:761877/76‑1 (MQ=255) ttcttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTa > 2:1403163/1‑76 (MQ=255) tcttcACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTAt > 1:901262/1‑76 (MQ=255) tcACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAg < 1:119319/76‑1 (MQ=255) tcACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAg > 2:871377/1‑76 (MQ=255) cacaCCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGt > 1:351421/1‑76 (MQ=255) cacCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCa < 2:345394/76‑1 (MQ=255) tataCACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCg > 1:844258/1‑76 (MQ=255) ataCACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGc > 1:1464399/1‑76 (MQ=255) ataCACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGc > 2:839410/1‑76 (MQ=255) ataCACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGc > 2:695687/1‑76 (MQ=255) ataCACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGc > 1:1464411/1‑76 (MQ=255) aGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGtgtg > 2:202065/1‑76 (MQ=255) aGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGtgtg > 2:189695/1‑76 (MQ=255) aGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGtgtg > 2:202075/1‑76 (MQ=255) ggCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa > 1:896062/1‑76 (MQ=255) ggCTATAAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGa < 2:805075/76‑1 (MQ=255) tataAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGa > 1:282211/1‑76 (MQ=255) ataAATGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaa < 1:1503708/76‑1 (MQ=255) aTGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaaaaa < 2:341801/76‑1 (MQ=255) aTGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGaaaaaa < 2:600864/76‑1 (MQ=255) tGATATAGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAAc > 1:603858/1‑76 (MQ=255) aGTGTTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGCCCCCCGCCAGGTGTGATGAAAAAACGATGtt < 1:602024/76‑1 (MQ=255) aGTGGTTATAGTTAGCGCGT‑TTTTTTATTATTAATCCGTATTAGCCGCCCGCCAGGTGTGACGAAAAAACGATGtt < 2:586399/76‑1 (MQ=255) aGTGGTTATAGTTAGCCCTT‑TTTTTTATTATTAAATCGTATTAGTCCCCCGCCAGGTGTGACGAAAAAACGATGtt < 1:996905/76‑1 (MQ=255) aGTGGTTATAGTTAGCACTT‑TTTTTTATTATTAAATCGTATTAGTCCCCCGCCAGGTGTGACGAAAAAACGATGtt < 1:383765/76‑1 (MQ=255) aGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCCCCCGCCAGGTATGACGAAAAAACGATGtt < 2:675446/76‑1 (MQ=255) aGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt < 2:974605/76‑1 (MQ=255) aGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt < 2:1051904/76‑1 (MQ=255) aGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt < 2:1159436/76‑1 (MQ=255) aGTGGTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGtt < 2:1048289/76‑1 (MQ=255) gTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGa < 2:1185065/76‑1 (MQ=255) gTTATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGa > 1:694054/1‑76 (MQ=255) ttATAGTTAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCCCCCGCCAGGTGTGACGAAAAAACGATGTTCTGAt < 1:1188010/76‑1 (MQ=255) tAGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCt > 2:299495/1‑76 (MQ=255) aGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTa < 2:282211/76‑1 (MQ=255) aGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTa < 2:1061718/76‑1 (MQ=255) aGCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTa > 1:477024/1‑76 (MQ=255) gCAC‑CTTTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTaa > 1:7409/1‑76 (MQ=255) | TTTTTTATTGCATATTATTTTTTCTTCACACCTATACACTAAGGCTATAAATGATATAGTGGTTATAGTTAGCAC‑C‑TTTTTTATTATTAAATCGTATTAGTCACCCGCCAGGTGTGACGAAAAAACGATGTTCTGATGGCGTCTAA > REL606/1332067‑1332212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |