Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,140,926	T→G	E107A (GAA→GCA)	ECB_02035 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,140,926	0	T	G	100.0%	159.2 / NA	39	E107A (GAA→GCA)	ECB_02035	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/27);  total (12/27)

ATCACAGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTTCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTAACAACTTGCACCCA  >  REL606/2140857‑2140996                                                                      |                                                                       aTCACAGCTTCTTCTATAACATTGGTCATTGTCGGCGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGgtg                                                                  <  2:326737/76‑1 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              >  2:1127977/1‑76 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              >  2:609207/1‑76 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              >  2:658936/1‑76 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              >  2:814792/1‑76 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              >  2:824146/1‑76 (MQ=255)     caGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGtata                                                              <  2:505493/76‑1 (MQ=255)       gCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACg                                                            <  1:617869/76‑1 (MQ=255)       gCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACg                                                            <  1:617884/76‑1 (MQ=255)         ttcttcTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGt                                                          <  2:1052700/76‑1 (MQ=255)            ttctATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAgg                                                       <  1:986034/76‑1 (MQ=255)               tataACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTGTACGGTAGGACa                                                    <  1:1201876/76‑1 (MQ=255)                ataACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGGCaa                                                   >  1:588160/1‑76 (MQ=255)                ataACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACaa                                                   >  1:423255/1‑76 (MQ=255)                  aaCATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAata                                                 >  2:751266/1‑76 (MQ=255)                  aaCATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAata                                                 <  1:40660/76‑1 (MQ=255)                    cATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAAtata                                               <  1:977239/76‑1 (MQ=255)                     aTTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGGCAATATaa                                              >  1:940427/1‑76 (MQ=255)                            aTTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCg                                       <  2:280438/76‑1 (MQ=255)                             ttGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGt                                      <  1:841500/76‑1 (MQ=255)                             ttGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGt                                      <  1:1126799/76‑1 (MQ=255)                              tGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTc                                     <  1:136588/76‑1 (MQ=255)                              tGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTc                                     >  2:488492/1‑76 (MQ=255)                               gTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCa                                    >  2:1340517/1‑76 (MQ=255)                                tCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCAt                                   <  2:1289999/76‑1 (MQ=255)                                tCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCAt                                   <  1:1461624/76‑1 (MQ=255)                                  gACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATcc                                 >  2:951599/1‑76 (MQ=255)                                  gACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATcc                                 <  2:629259/76‑1 (MQ=255)                                    cGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCt                               <  2:817228/76‑1 (MQ=255)                                      cTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCttt                             <  2:959943/76‑1 (MQ=255)                                      cTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCttt                             <  2:36563/76‑1 (MQ=255)                                      cTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCttt                             <  2:959924/76‑1 (MQ=255)                                      cTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCttt                             <  2:270671/76‑1 (MQ=255)                                       tGGCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTa                            <  2:915514/76‑1 (MQ=255)                                        ggCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTAc                           >  1:1114796/1‑76 (MQ=255)                                        ggCGCTTCTTCCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTAc                           <  1:217499/76‑1 (MQ=255)                                             ttcttcCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGttct                      <  2:1470955/76‑1 (MQ=255)                                               cttcCGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGttcttc                    <  1:617449/76‑1 (MQ=255)                                                   cGGGGCTTCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTa                >  1:209255/1‑76 (MQ=255)                                                          tCTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTAACAACtt         >  2:606312/1‑76 (MQ=255)                                                           cTGACTTCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTAACAACTTg        <  2:12808/76‑1 (MQ=255)                                                                 tCGCTGCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTAACAACTTGCACcca  <  2:1497962/76‑1 (MQ=255)                                                                      |                                                                       ATCACAGCTTCTTCTATAACATTGGTCATTGTCGACGCTGGCGCTTCTTCCGGGGCTTCTGACTTCGCTTCTGGTGTATACGGTAGGACAATATAAATGTTCGTCATCCCTTTACGTTCTTCACTAACAACTTGCACCCA  >  REL606/2140857‑2140996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑