Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,377,568	C→A	G74V (GGT→GTT)	rpsC ←	30S ribosomal protein S3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,377,568	0	C	A	100.0%	111.8 / NA	29	G74V (GGT→GTT)	rpsC	30S ribosomal protein S3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/13);  total (16/13)

GGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAC  >  REL606/3377496‑3377639                                                                         |                                                                        ggAACGCCAGGGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCg                                                                      <  1:720550/76‑1 (MQ=255)  gAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCgg                                                                     >  2:673466/1‑76 (MQ=255)   aaCGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCggg                                                                    >  2:820569/1‑76 (MQ=255)     cGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCg                                                                  <  2:749401/76‑1 (MQ=255)         aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCa                                                              >  2:502907/1‑76 (MQ=255)         aGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCa                                                              <  1:811718/76‑1 (MQ=255)             aTGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAgtgt                                                          >  2:1310396/1‑76 (MQ=255)               gTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGa                                                        >  2:120449/1‑76 (MQ=255)                  cGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATg                                                     >  1:1445727/1‑76 (MQ=255)                     tACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGtt                                                  >  1:1277094/1‑76 (MQ=255)                          ccTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACg                                             >  2:82715/1‑76 (MQ=255)                          ccTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACg                                             <  1:1435810/76‑1 (MQ=255)                            ttACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGa                                           >  1:995200/1‑76 (MQ=255)                             tACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGAt                                          >  2:224495/1‑76 (MQ=255)                             tACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGAt                                          >  2:1104032/1‑76 (MQ=255)                                gctgTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGct                                       <  2:947218/73‑1 (MQ=255)                                gCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGct                                       <  2:320044/76‑1 (MQ=255)                                    tttttCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTa                                   >  1:883689/1‑76 (MQ=255)                                    tttttCTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTa                                   <  2:736452/76‑1 (MQ=255)                                         cTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTAGACGGATGCTCTTAGCCgg                              <  2:578010/76‑1 (MQ=255)                                         cTACGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCgg                              >  1:669773/1‑76 (MQ=255)                                            cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg                           >  1:401026/1‑76 (MQ=255)                                            cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg                           <  1:1125725/76‑1 (MQ=255)                                            cGTCTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACg                           <  1:1065412/76‑1 (MQ=255)                                               cTTCACCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTc                        <  1:1149216/76‑1 (MQ=255)                                                   aCCTTTTTTACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATa                    <  1:401963/76‑1 (MQ=255)                                                          ttACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATAc             >  1:1354333/1‑76 (MQ=255)                                                           tACCGATAACGATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACg            >  2:1412689/1‑76 (MQ=255)                                                               gataacgataacCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGAt        <  1:963889/76‑1 (MQ=255)                                                               gataacgataacCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGAt        <  1:831554/76‑1 (MQ=255)                                                                     gATAACCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAc  <  2:508663/76‑1 (MQ=255)                                                                         |                                                                        GGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGAC  >  REL606/3377496‑3377639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑