| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 475,187 | C→T | G40D (GGT→GAT) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 475,187 | 0 | C | T | 97.4% | 140.9 / ‑3.4 | 39 | G40D (GGT→GAT) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (21/17); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
GCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCG > REL606/475114‑475255 | gCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACAtcc < 1:324308/76‑1 (MQ=255) gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTaa < 1:1373949/76‑1 (MQ=255) gTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTaa < 1:318695/76‑1 (MQ=255) ttCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAAc > 1:1465822/1‑76 (MQ=255) tCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACa > 2:1285581/1‑76 (MQ=255) cAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAg < 1:492233/76‑1 (MQ=255) aGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGa > 2:1268058/1‑76 (MQ=255) ccAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGata > 2:1351499/1‑76 (MQ=255) gTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATcc < 2:1510902/76‑1 (MQ=255) ttCCGGGGCAAGCTTGGTATCGGCAATAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATccc < 1:1413808/76‑1 (MQ=255) ttCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATccc > 2:1218189/1‑76 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCccacc < 2:1326095/76‑1 (MQ=255) cGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCccacc < 1:529831/76‑1 (MQ=255) cAAGCTGGGTAGCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAgagg < 2:922337/76‑1 (MQ=255) ttGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGaaa > 1:953049/1‑76 (MQ=255) tGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAAt < 1:958598/76‑1 (MQ=255) gTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATAc < 1:1016291/76‑1 (MQ=255) aTCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGCGGAGAAATACGt > 2:1158219/1‑76 (MQ=255) aTCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGt < 1:740812/76‑1 (MQ=255) cGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAg > 1:1223738/1‑76 (MQ=255) cGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAg > 1:720466/1‑76 (MQ=255) aacaaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTa < 2:1156887/76‑1 (MQ=255) acaaAGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTAt > 2:194426/1‑76 (MQ=255) aaGCCCGGAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTgg > 2:378822/1‑76 (MQ=255) ggAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCa > 2:106952/1‑76 (MQ=255) ggAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCa < 1:758113/76‑1 (MQ=255) ggAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCa > 2:696953/1‑76 (MQ=255) gAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCAt < 1:925651/76‑1 (MQ=255) gAGTGAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCAt < 1:462932/76‑1 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 2:1003279/1‑76 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 2:991475/1‑76 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 2:926919/1‑76 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 2:893512/1‑76 (MQ=255) gAATGGTCCTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCg > 2:119935/1‑76 (MQ=255) ccTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaa > 1:627793/1‑76 (MQ=255) cTGCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATaaa < 1:1351499/76‑1 (MQ=255) gCCAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAgg > 2:108868/1‑76 (MQ=255) ccAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGc > 1:416708/1‑76 (MQ=255) cAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCg < 1:52641/76‑1 (MQ=255) cAGCACATCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCg < 1:893837/76‑1 (MQ=255) | GCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGGTATCGGCAACAAAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGGCCAGCATGCCGAGGATAAAGGCG > REL606/475114‑475255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |