breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,691,8440TG66.7% 6.7 / 31.5 33N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6061,383,1570TG52.4% 7.6 / 27.5 21G224G (GGT→GGGycjXconserved protein with nucleoside triphosphate hydrolase domain
*REL6062,296,3290AC40.0% 76.9 / 31.9 55intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL606275,4180AC38.9% 55.5 / 12.6 36F528V (TTT→GTT) yagWpredicted receptor
*REL6064,393,0610TG30.2% 90.3 / 12.3 43V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6064,195,8740AC30.0% 96.9 / 10.2 50T318P (ACC→CCC) aceBmalate synthase
*REL6061,484,1050AG28.2% 71.3 / 14.1 39G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,747,9030GT27.4% 142.1 / 45.3 62A8S (GCA→TCA) ydiLhypothetical protein
*REL6063,284,6510CA23.3% 129.0 / 23.8 43G132W (GGG→TGG) mtgAmonofunctional biosynthetic peptidoglycan transglycosylase
*REL6061,875,4830GC22.7% 175.5 / 42.6 66intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6062,631,7720AC21.3% 118.9 / 11.0 47intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3120818 =48 (1.040)6 (0.140) 6/136 3.7 11.2% coding (586/2469 nt) yqiG predicted outer membrane usher protein
?REL606 3120837 = 52 (1.240)coding (605/2469 nt) yqiG predicted outer membrane usher protein
* ? REL606 = 369263157 (1.230)14 (0.370) 4/122 4.1 22.4% intergenic (‑111/+79) yiaY/selB predicted Fe‑containing alcohol dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
?REL606 = 3692640 51 (1.360)intergenic (‑120/+70) yiaY/selB predicted Fe‑containing alcohol dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
* ? REL606 4070923 =68 (1.470)4 (0.090) 4/142 4.7 6.1% coding (48/447 nt) frvA predicted enzyme IIA component of PTS
?REL606 4070947 = 59 (1.350)coding (24/447 nt) frvA predicted enzyme IIA component of PTS