breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 3,691,844 | 0 | T | G | 66.7% | 6.7 / 31.5 | 33 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 1,383,157 | 0 | T | G | 52.4% | 7.6 / 27.5 | 21 | G224G (GGT→GGG) | ycjX | conserved protein with nucleoside triphosphate hydrolase domain |
* | REL606 | 2,296,329 | 0 | A | C | 40.0% | 76.9 / 31.9 | 55 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 275,418 | 0 | A | C | 38.9% | 55.5 / 12.6 | 36 | F528V (TTT→GTT) | yagW | predicted receptor |
* | REL606 | 4,393,061 | 0 | T | G | 30.2% | 90.3 / 12.3 | 43 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,195,874 | 0 | A | C | 30.0% | 96.9 / 10.2 | 50 | T318P (ACC→CCC) | aceB | malate synthase |
* | REL606 | 1,484,105 | 0 | A | G | 28.2% | 71.3 / 14.1 | 39 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,747,903 | 0 | G | T | 27.4% | 142.1 / 45.3 | 62 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 3,284,651 | 0 | C | A | 23.3% | 129.0 / 23.8 | 43 | G132W (GGG→TGG) | mtgA | monofunctional biosynthetic peptidoglycan transglycosylase |
* | REL606 | 1,875,483 | 0 | G | C | 22.7% | 175.5 / 42.6 | 66 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 2,631,772 | 0 | A | C | 21.3% | 118.9 / 11.0 | 47 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 3120818 = | 48 (1.040) | 6 (0.140) | 6/136 | 3.7 | 11.2% | coding (586/2469 nt) | yqiG | predicted outer membrane usher protein |
? | REL606 | 3120837 = | 52 (1.240) | coding (605/2469 nt) | yqiG | predicted outer membrane usher protein | |||||
* | ? | REL606 | = 3692631 | 57 (1.230) | 14 (0.370) | 4/122 | 4.1 | 22.4% | intergenic (‑111/+79) | yiaY/selB | predicted Fe‑containing alcohol dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor |
? | REL606 | = 3692640 | 51 (1.360) | intergenic (‑120/+70) | yiaY/selB | predicted Fe‑containing alcohol dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor | |||||
* | ? | REL606 | 4070923 = | 68 (1.470) | 4 (0.090) | 4/142 | 4.7 | 6.1% | coding (48/447 nt) | frvA | predicted enzyme IIA component of PTS |
? | REL606 | 4070947 = | 59 (1.350) | coding (24/447 nt) | frvA | predicted enzyme IIA component of PTS |