| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 422,600 | (G)7→8 | intergenic (‑287/+175) | cyoA ← / ← ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 422,593 | 1 | . | G | 95.6% | 188.6 / 0.9 | 45 | intergenic (‑280/+182) | cyoA/ampG | cytochrome o ubiquinol oxidase subunit II/muropeptide transporter |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (19/24); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑GGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCAATA > REL606/422523‑422662 | gCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑ggggg < 2:102788/76‑1 (MQ=255) cAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑gggggg > 2:338074/1‑76 (MQ=255) aaTTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑ggggggg > 1:1633453/1‑76 (MQ=255) aTTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑gggggggg < 1:1613385/76‑2 (MQ=255) ttGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑gggggggga < 2:1416617/76‑3 (MQ=255) ataACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATa < 2:1351266/76‑1 (MQ=255) ataACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATa < 2:59104/76‑1 (MQ=255) ataACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATa < 2:494840/76‑1 (MQ=255) taACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATaa > 2:428075/1‑76 (MQ=255) taAACACTCTGGACTAAAACACTTTTTTAACAAATACTTATCATTCTTGTGACAATTGGG‑TAGGGGGAGCAAATaa > 2:428084/1‑76 (MQ=255) cAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGAc > 1:679675/1‑76 (MQ=255) aaTCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACt < 2:1150806/76‑1 (MQ=255) tCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCa > 2:36370/1‑76 (MQ=255) cTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCAt < 2:185198/76‑1 (MQ=255) tGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCAtt > 1:1363507/1‑76 (MQ=255) tGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCAtt > 1:1363496/1‑76 (MQ=255) ggAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTa < 2:295988/76‑1 (MQ=255) ggAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTa > 1:1134085/1‑76 (MQ=255) ggAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTa > 2:128357/1‑76 (MQ=255) aaTAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGc < 1:517163/76‑1 (MQ=255) aTAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGGAG‑GGGGGGGAAAATAAAGACTCATTAGCt > 2:1094498/1‑76 (MQ=255) aTAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCt > 1:1034344/1‑76 (MQ=255) tAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTg < 2:790757/76‑1 (MQ=255) tAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTg < 2:1422626/76‑1 (MQ=255) aaaaCAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGa > 1:262297/1‑76 (MQ=255) aaaCAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGaa > 2:931065/1‑76 (MQ=255) cAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCg < 2:571114/76‑1 (MQ=255) aaTTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgt < 2:956650/76‑1 (MQ=255) aTTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtg < 2:1625411/76‑1 (MQ=255) aTTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtg < 1:180063/76‑1 (MQ=255) ttGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCgtgt < 1:5264/76‑1 (MQ=255) tGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa < 2:1102288/76‑1 (MQ=255) tGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa < 2:638672/76‑1 (MQ=255) tGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa < 2:547935/76‑1 (MQ=255) tGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTa < 2:547926/76‑1 (MQ=255) tttAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAg < 1:1297245/76‑1 (MQ=255) tttAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAg < 2:774482/76‑1 (MQ=255) ttAACAAATAATTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGa > 1:587868/1‑76 (MQ=255) ataatTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATa > 2:367707/1‑76 (MQ=255) ataatTATCATTATGGTGACAATTGGG‑T‑GGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAg < 1:779190/76‑1 (MQ=255) aatTATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGa > 1:909860/1‑76 (MQ=255) ttATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAAt < 1:356559/76‑1 (MQ=255) ttATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAAt > 2:764373/1‑76 (MQ=255) tATCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAAtt > 1:819802/1‑76 (MQ=255) aTCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTc > 1:1439046/1‑76 (MQ=255) aTCATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTc < 1:1252362/76‑1 (MQ=255) cATTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCAt > 1:1568311/1‑76 (MQ=255) aTTATGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATa < 1:65807/76‑1 (MQ=255) tGGTGACAATTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAAtt > 2:1218177/1‑76 (MQ=255) aaTTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaa > 1:473812/1‑76 (MQ=255) aTTGGG‑TGGGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaat < 2:109417/76‑1 (MQ=255) atggg‑tgggGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCaata < 1:155450/75‑1 (MQ=255) | GCAATTGGTATAACCAATCTGGAATAAAACAATTGTTTAACAAATAATTATCATTATGGTGACAATTGGG‑T‑GGGGGGGAAAATAAAGACTCATTAGCTGAATCGTGTAAGAAAAAATAGAATTCATAAATTGCGTCAATA > REL606/422523‑422662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |