Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	100.0%	205.6 / NA	53	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (28/25);  total (28/25)

TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695                                                                            |                                                                       tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  1:1120499/1‑76 (MQ=255) tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  1:1120509/1‑76 (MQ=255) tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  1:1201247/1‑76 (MQ=255)  aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt                                                                      >  1:1442527/1‑76 (MQ=255)    cATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttt                                                                    <  2:1345723/76‑1 (MQ=255)     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt                                                                   >  2:346864/1‑76 (MQ=255)     attattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttt                                                                   <  1:466199/76‑1 (MQ=255)          tCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttttttca                                                              >  1:217941/1‑73 (MQ=255)               aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                          <  1:243817/76‑1 (MQ=255)                tACGAGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGa                                                         >  2:339805/1‑76 (MQ=255)                       ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                                  <  2:439282/76‑1 (MQ=255)                       ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                                  >  1:159956/1‑76 (MQ=255)                         tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACag                                                <  2:1506148/76‑1 (MQ=255)                          gACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACaga                                               >  1:1233838/1‑76 (MQ=255)                           aCTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACagag                                              >  2:1426559/1‑76 (MQ=255)                             tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGa                                            >  2:1033542/1‑76 (MQ=255)                              gTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGAt                                           <  1:8282/76‑1 (MQ=255)                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                          <  2:1398989/76‑1 (MQ=255)                               tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                          <  2:415692/76‑1 (MQ=255)                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         >  1:408224/1‑76 (MQ=255)                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         <  1:436121/76‑1 (MQ=255)                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         <  1:309635/76‑1 (MQ=255)                                aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         <  1:1305302/76‑1 (MQ=255)                                 aTTTGAGAACGAATTTAAA‑CGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAt                                       >  1:715597/1‑76 (MQ=255)                                   ttGAGAACGAAGTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAAtt                                      <  2:1365524/76‑1 (MQ=255)                                     gagaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGt                                    >  1:25009/1‑76 (MQ=255)                                      agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt                                   >  1:30600/1‑76 (MQ=255)                                      agaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGtt                                   >  1:636158/1‑76 (MQ=255)                                       gaACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTc                                  <  2:957464/76‑1 (MQ=255)                                             aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                            >  1:1112210/1‑76 (MQ=255)                                             aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAAc                            <  1:1480520/76‑1 (MQ=255)                                             aTTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCATGAAATGGACAGAGGATAAATTGTTCAATAAc                            >  1:314191/1‑76 (MQ=255)                                              tttAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACg                           <  2:318158/76‑1 (MQ=255)                                               ttAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg                          <  1:76923/76‑1 (MQ=255)                                               ttAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACgg                          >  1:840595/1‑76 (MQ=255)                                                tAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGa                         <  2:385238/76‑1 (MQ=255)                                                 aaaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        >  2:1046881/1‑76 (MQ=255)                                                 aaaCCGCTTCACCTGGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        <  2:820293/76‑1 (MQ=255)                                                  aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  1:1266584/76‑1 (MQ=255)                                                    ccGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                     >  2:707239/1‑76 (MQ=255)                                                      gCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc                   >  1:1127293/1‑76 (MQ=255)                                                       cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  <  1:346864/76‑1 (MQ=255)                                                       cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  >  1:1034710/1‑76 (MQ=255)                                                       cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  <  2:876112/76‑1 (MQ=255)                                                       cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  >  1:1238029/1‑76 (MQ=255)                                                        ttCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 >  1:329568/1‑76 (MQ=255)                                                        ttCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 <  1:600621/76‑1 (MQ=255)                                                         tCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                >  2:589945/1‑76 (MQ=255)                                                         tCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCg                >  1:563620/1‑76 (MQ=255)                                                           aCCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt              >  1:1348621/1‑76 (MQ=255)                                                              ttGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           <  1:206044/76‑1 (MQ=255)                                                              ttGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGAc           >  1:365024/1‑76 (MQ=255)                                                               tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          <  2:692135/76‑1 (MQ=255)                                                                gAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa         >  1:1635526/1‑76 (MQ=255)                                                                gAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGa         <  1:739435/76‑1 (MQ=255)                                                                  aaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       >  2:890743/1‑76 (MQ=255)                                                                   aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      >  2:1456668/1‑76 (MQ=255)                                                                   aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      <  1:1329350/76‑1 (MQ=255)                                                                   aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      <  1:220877/76‑1 (MQ=255)                                                                    aaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgc     >  2:1230567/1‑76 (MQ=255)                                                                       cTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc  >  1:1336171/1‑76 (MQ=255)                                                                            |                                                                       TAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875551‑3875695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑