Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,165,828	A→C	I290L (ATC→CTC)	rpoC →	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,165,828	0	A	C	100.0%	231.8 / NA	55	I290L (ATC→CTC)	rpoC	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/20);  total (35/20)

AACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGGTCGTCGCGGT  >  REL606/4165756‑4165899                                                                         |                                                                        aaCGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcctca                                                                      >  2:1030677/1‑76 (MQ=255)  aCGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcctcat                                                                     <  1:549956/76‑1 (MQ=255)   cGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACctcatc                                                                    <  1:1106066/76‑1 (MQ=255)   cGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACctcatc                                                                    <  1:1588144/76‑1 (MQ=255)    gATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcg                                                                   <  1:1618046/76‑1 (MQ=255)     aTCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgt                                                                  <  1:404952/76‑1 (MQ=255)     aTCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgt                                                                  >  1:1624210/1‑76 (MQ=255)     aTCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgt                                                                  >  1:1458098/1‑76 (MQ=255)         gTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgtacgt                                                              >  2:1498410/1‑76 (MQ=255)          tATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgtacgta                                                             >  2:1038703/1‑76 (MQ=255)          tATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgtacgta                                                             >  2:1497121/1‑76 (MQ=255)          tATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATcgtacgta                                                             >  2:611268/1‑76 (MQ=255)           aTCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTaa                                                            >  2:142353/1‑76 (MQ=255)            tcgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAAc                                                           <  1:691481/76‑1 (MQ=255)             cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACg                                                          <  1:294953/76‑1 (MQ=255)             cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACg                                                          <  1:623665/76‑1 (MQ=255)              gtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGa                                                         >  1:1221397/1‑76 (MQ=255)                cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGaaa                                                       >  1:99362/1‑76 (MQ=255)                cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGaaa                                                       <  1:65683/76‑1 (MQ=255)                 gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGacaa                                                      >  1:793597/1‑76 (MQ=255)                     cATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGt                                                  >  1:232022/1‑76 (MQ=255)                       ttAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTAt                                                <  1:414547/76‑1 (MQ=255)                        tAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTAtg                                               <  1:1294710/76‑1 (MQ=255)                        tAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTAtg                                               >  1:265393/1‑76 (MQ=255)                              tAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCCTACGTAACGAAAAACGTATGCTGCAg                                         >  1:43153/1‑76 (MQ=255)                               aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAgg                                        <  1:1667331/76‑1 (MQ=255)                                acaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGa                                       >  2:1406711/1‑76 (MQ=255)                                acaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGa                                       >  2:62668/1‑76 (MQ=255)                                  aacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAg                                     <  1:1444039/76‑1 (MQ=255)                                    ccGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCg                                   <  1:73652/76‑1 (MQ=255)                                     cGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCgg                                  >  2:77690/1‑76 (MQ=255)                                     cGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCgg                                  >  2:5183/1‑76 (MQ=255)                                      gTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGt                                 <  2:1544135/76‑1 (MQ=255)                                       tCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGtt                                >  1:1573290/1‑76 (MQ=255)                                       tCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGtt                                <  2:190502/76‑1 (MQ=255)                                        cTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTg                               >  2:555011/1‑76 (MQ=255)                                        cTGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTg                               >  2:468531/1‑76 (MQ=255)                                         tGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGa                              >  2:432777/1‑76 (MQ=255)                                         tGAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGa                              >  1:681324/1‑76 (MQ=255)                                          gAAACGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGGTGAc                             >  1:972467/1‑76 (MQ=255)                                           aaaCGTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACg                            <  1:35070/76‑1 (MQ=255)                                               gTCTGCTGGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCct                        <  1:193489/76‑1 (MQ=255)                                                 ctgctgGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCccgc                      >  1:541632/1‑76 (MQ=255)                                                  tgctgGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCctgct                     <  2:902273/76‑1 (MQ=255)                                                   gctgGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCctgctg                    >  1:304925/1‑76 (MQ=255)                                                   gctgGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCctgctg                    <  2:827407/76‑1 (MQ=255)                                                   gctgGATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGGTGACGCCctgccg                    >  2:809691/1‑74 (MQ=255)                                                      ggATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGGGGTTGACGCCCTGCTggct                 >  2:756812/1‑74 (MQ=255)                                                      ggATCTGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGAt                 >  2:495132/1‑76 (MQ=255)                                                           tGGCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACgg            <  1:342238/76‑1 (MQ=255)                                                            ggCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgt           <  1:1305588/76‑1 (MQ=255)                                                            ggCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgt           >  2:1002114/1‑76 (MQ=255)                                                            ggCTGCCCCGGACCTCAACGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgt           <  2:133411/76‑1 (MQ=255)                                                             gCTGCGCCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgtc          >  1:1356304/1‑76 (MQ=255)                                                                gcgcCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgtcgtc       >  2:128347/1‑76 (MQ=255)                                                                gcgcCGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgtcgtc       >  2:900126/1‑76 (MQ=255)                                                                gcgcCGGACCTAATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGgtcgtc       <  1:1301552/76‑1 (MQ=255)                                                                   ccGGACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGGTCGTcgcg    >  2:108220/1‑76 (MQ=255)                                                                    cGGACCTCATCGTACGTAACGAAAAACGTATGCTGCATGAAGCGGTTGACGCCGNGCTGGATAACGGTCGTCGCgg   >  2:267991/1‑76 (MQ=255)                                                                     ggACCTCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGGTCGTCGCGGt  >  2:471562/1‑76 (MQ=255)                                                                         |                                                                        AACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTTGACGCCCTGCTGGATAACGGTCGTCGCGGT  >  REL606/4165756‑4165899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑