| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,179,865 | A→C | intergenic (+159/‑28) | yjaG → / → hupA | hypothetical protein/HU, DNA‑binding transcriptional regulator, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,179,865 | 0 | A | C | 98.2% | 238.5 / ‑4.1 | 56 | intergenic (+159/‑28) | yjaG/hupA | hypothetical protein/HU, DNA‑binding transcriptional regulator, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (21/34); total (21/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
AAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCAG > REL606/4179792‑4179935 | aaaaaaGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGc < 1:531280/76‑1 (MQ=255) aaaaGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGa < 2:80108/76‑1 (MQ=255) aaaGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGAt > 2:500077/1‑76 (MQ=255) aaaGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGAt > 1:889832/1‑76 (MQ=255) aGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATaa < 1:380367/76‑1 (MQ=255) gTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATaaa < 2:1213458/76‑1 (MQ=255) gTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATaaa < 1:339083/76‑1 (MQ=255) tGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAac > 2:594602/1‑76 (MQ=255) ggCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAaca < 1:1524626/76‑1 (MQ=255) cTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAAcaca < 2:237912/76‑1 (MQ=255) tATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACAt > 2:907519/1‑76 (MQ=255) ggTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTa > 2:833049/1‑76 (MQ=255) ggTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTa > 2:1380707/1‑76 (MQ=255) ggTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTa < 2:604580/76‑1 (MQ=255) gTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTaa < 1:566653/76‑1 (MQ=255) tGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAg < 2:479295/76‑1 (MQ=255) gCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAgg > 1:1043706/1‑76 (MQ=255) gtgtATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGAt < 1:1659864/76‑1 (MQ=255) gtATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATaa > 1:1453630/1‑76 (MQ=255) tATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAAc > 2:620905/1‑76 (MQ=255) tATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAAc < 2:586749/76‑1 (MQ=255) aTGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACt > 2:1640922/1‑76 (MQ=255) aTGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACt > 1:250889/1‑76 (MQ=255) tGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACtt < 2:665330/76‑1 (MQ=255) tGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACtt < 2:1411086/76‑1 (MQ=255) tGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACtt < 2:1347167/76‑1 (MQ=255) gCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTa < 1:1133016/76‑1 (MQ=255) cAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTAt < 2:1504847/76‑1 (MQ=255) aGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATg < 1:581901/76‑1 (MQ=255) aGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATg < 1:1318179/76‑1 (MQ=255) agagTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAAc > 1:1381699/1‑76 (MQ=255) gagTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACa < 1:1465820/76‑1 (MQ=255) tGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGa < 2:588149/76‑1 (MQ=255) tATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTc > 1:1193879/1‑76 (MQ=255) tATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTc > 2:320242/1‑76 (MQ=255) tATTCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTc > 2:1063839/1‑76 (MQ=255) aTTCTGTCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCa < 1:83726/76‑1 (MQ=255) ttCTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCaa < 2:1073140/76‑1 (MQ=255) cTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACt < 2:1585575/76‑1 (MQ=255) cTGGCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACt < 2:1478356/76‑1 (MQ=255) ggCATTTCCGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAAAtga > 1:90336/1‑76 (MQ=255) cGTCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTa < 1:1578494/76‑1 (MQ=255) tCGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAAt < 1:111289/76‑1 (MQ=255) cGCACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAAtt < 2:264394/76‑1 (MQ=255) cACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGc < 2:634777/76‑1 (MQ=255) cACTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGc < 1:214325/76‑1 (MQ=255) aCTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACCCAACTGATTGATGTAATTGCa > 2:398871/1‑76 (MQ=255) cTCGATGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCag < 2:250889/76‑1 (MQ=255) aTGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGaa < 2:510996/76‑1 (MQ=255) aTGCTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGaa > 2:323448/1‑76 (MQ=255) cTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGc < 1:154234/76‑1 (MQ=255) cTTAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGc > 1:955796/1‑76 (MQ=255) ttAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCa > 1:474615/1‑76 (MQ=255) ttAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCa > 2:161978/1‑76 (MQ=255) ttAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCa < 1:1656408/76‑1 (MQ=255) tAGCACGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCAg < 1:1094401/76‑1 (MQ=255) | AAAAAAGTGGCTATCGGTGCGTGTATGCAGGAGAGTGCTATTCTGGCATTTCCGTCGCACTCGATGCTTAGCAAGCGATAAACACATTGTAAGGATAACTTATGAACAAGACTCAACTGATTGATGTAATTGCAGAGAAAGCAG > REL606/4179792‑4179935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |