Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,252,012	G→A	A250V (GCT→GTT)	nusA ←	transcription elongation factor NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,252,012	0	G	A	100.0%	138.4 / NA	36	A250V (GCT→GTT)	nusA	transcription elongation factor NusA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG  >  REL606/3251939‑3252084                                                                          |                                                                         gACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACa                                                                        <  1:1454758/76‑1 (MQ=255)    gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCt                                                                     <  2:677055/76‑1 (MQ=255)      tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTAc                                                                   <  2:787902/76‑1 (MQ=255)       atCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTAcc                                                                  >  1:289750/1‑76 (MQ=255)         cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCgg                                                                >  1:1452543/1‑76 (MQ=255)          gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGa                                                               <  2:159716/76‑1 (MQ=255)           aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGAt                                                              >  1:1599953/1‑76 (MQ=255)            tACCCTCGCCACCCAGTTCAGCAGACACCGCCTGAACGCGACCGCCACGCATACGCACGAAAACACCTACCGGacc                                                             >  2:283180/1‑74 (MQ=255)                cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATa                                                         <  1:146238/76‑1 (MQ=255)                cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATa                                                         <  2:163615/76‑1 (MQ=255)                  cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACg                                                       <  1:1328719/76‑1 (MQ=255)                     cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGttt                                                    >  2:1343401/1‑76 (MQ=255)                     cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGttt                                                    >  1:1572941/1‑76 (MQ=255)                      aCCCAGTTCAGTAGACACCGCNGGAACACGCGCGCCACTCATACCTACGCAAACACCTACCGGATCGATACGTTTa                                                   <  2:279591/76‑1 (MQ=255)                       cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTAt                                                  <  2:517187/76‑1 (MQ=255)                       cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTAt                                                  >  1:544682/1‑76 (MQ=255)                        ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATc                                                 <  2:1113947/76‑1 (MQ=255)                          aGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGt                                               >  2:1009550/1‑76 (MQ=255)                           gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGtt                                              >  1:984681/1‑76 (MQ=255)                           gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGtt                                              >  1:45091/1‑76 (MQ=255)                              cAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGt                                           >  2:241063/1‑76 (MQ=255)                                 tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGtttt                                        <  2:54295/76‑1 (MQ=255)                                  aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTc                                       <  2:1594241/76‑1 (MQ=255)                                   gACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCa                                      >  1:630942/1‑76 (MQ=255)                                    acacCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCAc                                     >  2:1385739/1‑76 (MQ=255)                                            tGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt                             >  2:457891/1‑76 (MQ=255)                                             gAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt                            >  1:1345205/1‑76 (MQ=255)                                                 acGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCa                        <  1:111967/76‑1 (MQ=255)                                                   gcgcgcCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg                      <  2:801692/76‑1 (MQ=255)                                                      cgcCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga                   >  1:753395/1‑76 (MQ=255)                                                           cGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg              <  2:1175053/76‑1 (MQ=255)                                                             cATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGa            <  2:358320/76‑1 (MQ=255)                                                              aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt           <  1:888804/76‑1 (MQ=255)                                                              aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt           <  2:299994/76‑1 (MQ=255)                                                                aCCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg         >  2:160540/1‑76 (MQ=255)                                                                   tACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa      >  2:11718/1‑76 (MQ=255)                                                                       cAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg  <  2:45091/76‑1 (MQ=255)                                                                       cAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg  <  2:984681/76‑1 (MQ=255)                                                                          |                                                                         GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG  >  REL606/3251939‑3252084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑